LOCUS       sequence-223036-        3280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - GreenRenilla Luciferase CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: pGrRenilla
FEATURES             Location/Qualifiers
     source          1..3280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(569..1228)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1143..1162
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1229..1331)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1446..2034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1935..1954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2149..2165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2149..2165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2181..2199
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2181..2198
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3219..3238)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3220..3238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3245..3262)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3245..3261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgctggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtaaca tcatgtttga cagcttatca
      481 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
      541 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
      601 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
      661 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
      721 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
      781 agggattggc tgacacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
      841 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
      901 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
      961 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     1021 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     1081 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     1141 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     1201 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     1261 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     1321 cgtgccgatc atcacaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1381 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1441 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1501 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1561 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1621 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1681 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1741 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1801 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1861 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1921 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1981 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2041 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2101 cctgcgttat cccctgattc tgtggataac cgtgtcatga tgcctggaca ggaaacagct
     2161 atgaccaacg gctctatggt atttaggtga cactatagaa cgaattcact agtgattggg
     2221 cgatgagtgg tctcgaatgg cttccaaagt ctacgaccca gaacaaagga agaggatgat
     2281 tactggaccg cagtggtggg caaggtgcaa gcagatgaat gtactcgata gttttatcaa
     2341 ttattacgac tccgaaaaac acgcggaaaa tgctgtcata ttcctgcacg ggaacgccac
     2401 tagcagctac ttgtggcggc acgtagtccc ccacatagag cctgttgccc gatgtattat
     2461 tcccgatctc attggaatgg gcaaatcagg caaatctggg aacggttctt accgcctgct
     2521 cgatcactat aaatatttga ccgcttggtt tgagctgctt aatctcccta aaaaaataat
     2581 attcgttgga cacgactggg gctcagctct ggcgttccac tacgcatacg agcatcaaga
     2641 ccgaataaaa gcaatagtac atatggagtc agttgttgac gtcatagagt catggatggg
     2701 ttggccagat attgaagagg aattggccct catcaagagt gaggaggggg aaaaaatggt
     2761 cctggaaaat aattttttcg tggaaacctt gctcccgagt aaaataatgc gaaaacttga
     2821 acccgaagag tttgctgctt atttggagcc tttcaaggaa aagggggaag taagacgacc
     2881 aaccctttct tggccgaggg agatacctct tgttaagggt ggaaagcccg acgtagtcca
     2941 gatagtccga aattacaacg cgtacctgag agctagtgat gacctcccta agttgtttat
     3001 cgagtcagac cctgggttct tcagcaacgc aattgtagaa ggagcgaaaa aatttccaaa
     3061 tacagaattt gtgaaggtta aggggctcca ctttttgcaa gaggacgccc ccgatgagat
     3121 gggaaaatac attaaatctt tcgtagagcg cgtccttaag aatgaacagt gagcttcgag
     3181 accttgagat caatatctaa ggtcctaagc ggaattcacc ctatagtgag tcgtattaca
     3241 attcactggc cgtcgtttta catgcgtttc tacaatgtta
//
