LOCUS       sequence-223038-        2843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - Cytomegalovirus enhancer and promoter for constitutive high
            level expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: phCMV-IE1
FEATURES             Location/Qualifiers
     source          1..2843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     587..606
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(825..929)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1028..1050
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1042..1059
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1043..1059
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        1185..1488
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1489..1692
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1643..1663
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(1790..1806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1790..1806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1803..1825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1978..2566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2058..2077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2683..2707)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2807..2843,1..755,756..824))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
       61 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagagcc acgctcaccg
      121 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      181 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      241 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      301 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
      361 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      421 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      481 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      541 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      601 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      661 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      721 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      781 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      841 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      901 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc agatacctga
      961 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
     1021 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
     1081 tggaataagg gtcaatctgc cgcaatccag tctggtctca ggagtagtta ttaatagtaa
     1141 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1201 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1261 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1321 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     1381 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     1441 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     1501 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     1561 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     1621 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     1681 ataagcagag ctggtttagt gaaccgtcag atcaagctca aatgagagac cactggttat
     1741 ctcggcacag acggatgata agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt
     1801 ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc tatccgacaa
     1861 tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg gaaagaacat
     1921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2581 cttttctacg gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa gtcagcgtaa
     2641 atgggtaggg ggcttcaaat cgtcctcgtg ataccaattc ggagcctgct tttttgtaca
     2701 aacttgttga taatggcaat tcaaggatct tcacctagat ccttttaaat taaaaatgaa
     2761 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2821 tcagtgaggc acctatctca gcg
//
