LOCUS       sequence-223046-        6759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ITRACKgamma Donor Construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Colgan LA, Hu M, Misler JA, Parra-Bueno P, Moran CM, Leitges M, 
            Yasuda R
  TITLE     PKCalpha integrates spatiotemporally distinct Ca(2+) and autocrine 
            BDNF signaling to facilitate synaptic plasticity.
  JOURNAL   Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 
            10.1038/s41593-018-0184-3. Epub 2018 Jul 16.
  PUBMED    30013171
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-018-0184-3"; journalName: "Nat Neurosci"; date: 
            "2018-08"; volume: "21"; issue: "8"; pages: "1027-1037"
COMMENT     Sequence Label: CMV-mEGFP-PKCg-C1
FEATURES             Location/Qualifiers
     source          1..6759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      597..606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(603..605,606..608,609..1121)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(648..669)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(909..928)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1256..1277
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3547..3668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3584..3603)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3638..3657
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3675..4130)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3812..3833)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4024..4043
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4157..4261
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4263..4620
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4263..4454
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4286..4306)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4471..4606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4655..5449
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4655..5449
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4709..4728)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5319..5338
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5637..5656)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5681..5728
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6057..6645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6546..6565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1021 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1081 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1201 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg cgtcgacggt accgcgggcc
     1321 cgggatccgg aatggcgggt ctgggccctg gcggaggcga ctcagagggg ggaccccgac
     1381 ccctgttttg cagaaagggg gcgctgaggc agaaggtggt ccacgaggtg aagagccaca
     1441 agttcaccgc tcgtttcttc aagcagccaa ccttctgcag tcactgtacc gacttcatct
     1501 ggggcattgg aaagcagggc ctgcaatgtc aagtctgtag ctttgtggtt caccgccgat
     1561 gccacgaatt tgtgaccttc gagtgtccag gcgctggaaa gggcccccag acggacgacc
     1621 ctcgcaacaa gcacaagttc cgtctgcaca gctacagcag tcccaccttc tgcgaccact
     1681 gtggctccct cctttacggg ctggtgcacc agggcatgaa atgttcctgt tgcgagatga
     1741 atgtgcaccg gcgctgtgtg cgcagcgtgc cctccctttg cggtgtggac cacacagagc
     1801 gccgtggacg tctgcaactg gaaatccggg ctcctacgtc ggatgagatc catattactg
     1861 ttggcgaggc ccggaacctc attcctatgg accccaatgg cctgtctgat ccctacgtga
     1921 aactgaagct gatcccggac cctcggaacc tgacaaaaca gaagacaaag accgtgaaag
     1981 ccacactgaa tcccgtgtgg aacgagacct tcgtgttcaa cctgaagcca ggggatgtag
     2041 agcgccggct cagtgtggag gtgtgggatt gggataggac atcccgaaat gacttcatgg
     2101 gtgccatgtc ctttggtgtc tcagagctac tcaaggcccc tgtggatgga tggtacaagt
     2161 tactgaacca ggaggagggc gaatattaca atgtaccggt ggccgatgct gacaactgca
     2221 gcctcctcca gaagtttgag gcctgcaatt accccttgga attgtatgag agagtgcgga
     2281 tgggcccctc ttcctccccc atcccttctc catcccccag tcctacggac tccaagcgat
     2341 gcttctttgg tgccagccca ggacgcctgc atatctctga cttcagcttc ctcatggttc
     2401 tagggaaagg cagttttggg aaggtaatgc tggcagagcg cagaggctcc gacgaactct
     2461 atgccatcaa gatactgaag aaagacgtca tcgtccagga tgacgatgta gactgcaccc
     2521 tcgtagagaa gcgtgtcctg gcattgggag gccgaggtcc tggaggccgg ccacactttc
     2581 tcacgcagct tcactccacc tttcagactc cggaccgcct gtattttgtg atggagtatg
     2641 tcactggggg cgatttaatg taccacatcc agcaactggg caagtttaag gagcctcatg
     2701 cagcattcta cgctgcggaa atcgccatag gcctcttctt ccttcacaac cagggcatca
     2761 tctacaggga cctcaagttg gataatgtga tgctggatgc tgaaggacac atcaagatca
     2821 cagactttgg catgtgtaaa gagaatgtct tccctgggtc cacaacccgc accttctgtg
     2881 gcaccccaga ctacatagca cctgagatca ttgcctatca gccctacggg aagtctgtcg
     2941 actggtggtc ttttggggtc ctgctgtatg agatgttggc aggacagcca ccctttgatg
     3001 gggaagatga ggaggagttg tttcaagcca tcatggaaca aactgtcacc tatcccaagt
     3061 cactttcccg ggaagctgtg gccatctgca aagggttcct gaccaagcac ccaggaaaac
     3121 gcctgggctc agggccagat ggggaaccca ccatccgggc tcatggcttt ttccgttgga
     3181 tcgattggga gaggttggag agactggaaa ttgcacctcc tttcagacca cgtccgtgtg
     3241 gccgcagtgg cgaaaacttt gacaagttct tcacgcgggc agcgccagca ctgaccccgc
     3301 cagaccgctt ggttctagcc agcatcgacc aagctgattt ccagggcttt acttatgtga
     3361 acccggactt cgtgcaccca gatgcccgca gccccacaag ccctgtgccc gtgcctgtga
     3421 tgtaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     3481 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3541 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3601 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3661 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     3721 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3781 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3841 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3901 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     3961 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4021 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4081 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     4141 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4201 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4261 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4321 ggctccccag cgggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4441 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4501 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4561 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4621 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     4681 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4741 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4801 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     4861 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4921 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4981 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5041 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5101 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5161 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5221 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5281 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5341 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5401 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     5461 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     5521 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     5581 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     5641 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     5701 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     5761 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     5821 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     5881 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     5941 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6001 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6061 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6121 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6181 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6241 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6301 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6361 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6421 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6481 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6541 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6601 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6661 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6721 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
