LOCUS       sequence-223069-        6917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of truncated Rag1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Grundy GJ, Yang W, Gellert M
  TITLE     Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome
            by an epigenetic signal in V(D)J recombination.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22487-92. doi: 
            10.1073/pnas.1014958107. Epub 2010 Dec 13.
  PUBMED    21149691
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1014958107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-12-28- 28"; volume: "107"; issue: "52"; pages: 
            "22487-92"
COMMENT     Sequence Label: coreR1 (384-1008)
FEATURES             Location/Qualifiers
     source          1..6917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     432..454
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     516..535
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      584..593
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             596..619
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             626..1723
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1580..1589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1697..1720
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1772..1795
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(3776..3795)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3841..3896
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3895..3914)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4257..4273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4257..4273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4270..4292)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4281..4297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4305..4311,4312..4329,4330..4335))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4350..4371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4500..4517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4671..5259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4751..4770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5430..6221,6222..6290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6053..6072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6291..6395)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6426..6805
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
       61 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
      121 ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
      181 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
      241 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
      301 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
      361 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
      421 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
      481 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
      541 tcatcatttt ggcaaagaat tgcggccgtc tcaggccgaa ttcgccacca tggtgcatca
      601 ccatcaccat caccatcatc gatccatgat cgaagaaggt aaactggtaa tctggattaa
      661 cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg
      721 aattaaagtc accgttgagc atccggataa actggaagag aaattcccac aggttgcggc
      781 aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca
      841 atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt
      901 tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc
      961 gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat
     1021 cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca
     1081 agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga
     1141 aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct
     1201 gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat
     1261 cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg
     1321 gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg
     1381 tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa
     1441 caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc
     1501 ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc
     1561 gaaagatcca cgtattgccg ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa
     1621 catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag
     1681 cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actaattcga gctcgaacaa
     1741 caacaacaat aacaataaca acaacctcgg gctcgaagtc ctattccagg gaccggtgca
     1801 tatcaataaa gggggacggc ctcgccagca tctcctgtca ctgacgagaa gggcgcagaa
     1861 acatcggctg agggagctca agattcaagt caaagaattt gctgacaaag aagaaggtgg
     1921 agatgtgaag gctgtctgct tgacattgtt tctcctggca ctgagggcga ggaatgagca
     1981 caggcaagct gatgaattag aggccatcat gcaaggcagg ggctccgggc ttcaaccagc
     2041 tgtttgcttg gccatccgtg tcaatacctt cctcagctgt agccaatacc ataagatgta
     2101 caggactgtg aaagctatca ctgggaggca gatttttcaa cctctgcatg ctcttcggaa
     2161 tgccgagaaa gtccttctgc caggctacca tccctttgag tggcagcccc cactgaagaa
     2221 tgtgtcctcc agaactgatg ttggaattat tgatgggctg tctggacttg cctcctctgt
     2281 ggatgagtac ccagtagata ccattgcgaa gaggttccgc tacgactctg ctttggtgtc
     2341 tgctttgatg gacatggaag aagacatctt ggaaggcatg agatcccaag atcttgatga
     2401 ctacctgaat ggtcccttca cagtggtggt aaaggagtct tgcgatggaa tgggggatgt
     2461 gagtgagaag cacgggagtg ggcccgcagt tccagaaaag gccgttcgtt tctctttcac
     2521 agtcatgaga attacgatag agcatggttc acagaacgtg aaggtgtttg aggaacccaa
     2581 gcccaattct gaactgtgtt gcaagccgtt gtgtcttatg ctggcagatg agtctgacca
     2641 tgagaccctt actgctattc taagccccct cattgccgag agggaggcca tgaagagcag
     2701 tgaattaacg ctggagatgg gaggcatccc caggactttt aaattcatct tcaggggcac
     2761 tggatacgat gaaaaacttg tccgggaagt agaaggcttg gaagcttctg gctcagtcta
     2821 catctgtaca ctctgtgaca ccacccgttt ggaagcctct cagaatcttg tcttccactc
     2881 cataaccaga agccacgccg agaacctgca gcgctatgag gtctggcggt ccaatccgta
     2941 tcatgagtcc gtggaagagc tccgggaccg ggtgaaaggg gtctctgcca aacctttcat
     3001 cgagacagtc ccttccatag atgcgcttca ctgtgacatt ggcaatgcag ctgaattcta
     3061 taagattttc cagctggaga taggggaagt gtataaacat cccaatgcct ctaaagagga
     3121 aaggaagaga tggcaggcca cgctggacaa acatctccgg aaaaggatga acttaaaacc
     3181 aatcatgagg atgaatggca actttgcccg gaagcttatg acccaagaga ctgtagacgc
     3241 agtttgtgag ttaattcctt ctgaggagag gcatgaagct ctcagggagc tcatggacct
     3301 ttacctgaag atgaaacccg tgtggcgctc ttcatgtccc gctaaagagt gtccagagtc
     3361 cctctgtcag tacagtttca actcacagcg tttcgcggaa ctcctctcca ccaagttcaa
     3421 atatagatac gagggcaaaa tcaccaatta ctttcacaaa accttggcac atgtccctga
     3481 aattattgaa agggatggct ctatcggggc ctgggcaagt gagggaaatg aatcgggtaa
     3541 caagctgttt agacggtttc ggaaaatgaa tgccaggcag tccaagtgct atgagatgga
     3601 agatgtcctg aaacatcact ggctgtatac ttcaaaatac ctccagaagt ttatgaatgc
     3661 tcataacgcg taactcgaga ctagtatcgc gataattcac tcctcaggtg caggctgcct
     3721 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
     3781 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     3841 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     3901 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     3961 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     4021 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     4081 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     4141 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     4201 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca
     4261 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4321 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4381 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatcga tccgctgcat
     4441 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4501 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4561 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4621 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5101 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5341 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6421 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     6481 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     6541 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     6601 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     6661 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     6721 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     6781 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     6841 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     6901 agcgatgggg gcggggg
//
