LOCUS       sequence-223072-        6605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of truncated Rag2 including the C-terminal region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Grundy GJ, Yang W, Gellert M
  TITLE     Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome
            by an epigenetic signal in V(D)J recombination.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22487-92. doi: 
            10.1073/pnas.1014958107. Epub 2010 Dec 13.
  PUBMED    21149691
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1014958107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-12-28- 28"; volume: "107"; issue: "52"; pages: 
            "22487-92"
COMMENT     Sequence Label: R2Ct (1-520)
FEATURES             Location/Qualifiers
     source          1..6605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     432..454
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     516..535
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      584..593
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             596..619
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             626..1723
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1580..1589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1697..1720
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1772..1795
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(3464..3483)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3529..3584
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3583..3602)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3945..3961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3945..3961)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3958..3980)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3969..3985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3993..3999,4000..4017,4018..4023))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4038..4059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4188..4205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4359..4947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4439..4458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5118..5909,5910..5978))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5741..5760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5979..6083)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6114..6493
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
       61 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
      121 ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
      181 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
      241 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
      301 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
      361 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
      421 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
      481 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
      541 tcatcatttt ggcaaagaat tgcggccgtc tcaggccgaa ttcgccacca tggtgcatca
      601 ccatcaccat caccatcatc gatccatgat cgaagaaggt aaactggtaa tctggattaa
      661 cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg
      721 aattaaagtc accgttgagc atccggataa actggaagag aaattcccac aggttgcggc
      781 aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca
      841 atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt
      901 tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc
      961 gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat
     1021 cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca
     1081 agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga
     1141 aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct
     1201 gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat
     1261 cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg
     1321 gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg
     1381 tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa
     1441 caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc
     1501 ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc
     1561 gaaagatcca cgtattgccg ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa
     1621 catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag
     1681 cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actaattcga gctcgaacaa
     1741 caacaacaat aacaataaca acaacctcgg gctcgaagtc ctattccagg gaccggtgtc
     1801 cctgcagatg gtaacagtgg gtcataacat agccttaatt caaccaggct tctcacttat
     1861 gaattttgat ggccaagttt tcttctttgg ccagaaaggc tggcctaaga gatcctgtcc
     1921 tactggagtc tttcattttg atataaaaca aaatcatctc aaactgaagc ctgcaatctt
     1981 ctctaaagat tcctgctacc tcccacctct tcgttatcca gctacttgct catacaaagg
     2041 cagcatagac tctgacaagc atcaatatat cattcacgga gggaaaacac caaacaatga
     2101 gctttccgat aagatttata tcatgtctgt cgcttgcaag aataacaaaa aagttacttt
     2161 ccgttgcaca gagaaagact tagtaggaga tgtccctgaa cccagatacg gccattccat
     2221 tgacgtggtg tatagtcgag ggaaaagcat gggtgttctc tttggaggac gttcatacat
     2281 gccttctacc cagagaacca cagaaaaatg gaatagtgta gctgactgcc taccccatgt
     2341 tttcttgata gattttgaat ttgggtgtgc tacatcatat attctcccag aacttcagga
     2401 tgggctgtct tttcatgttt ctattgccag aaacgatacc gtttatattt tgggaggaca
     2461 ctcacttgcc agtaatatac gccctgctaa cttgtataga ataagagtgg accttcccct
     2521 gggtacccca gcagtgaatt gcacagtctt gccaggagga atctctgtct ccagtgcaat
     2581 cctcactcaa acaaacaatg atgaatttgt tattgtgggt ggttatcagc tggaaaatca
     2641 gaaaaggatg gtctgcagcc ttgtctctct aggggacaac acgattgaaa tcagtgagat
     2701 ggagactcct gactggacct cagatattaa gcatagcaaa atatggtttg gaagcaacat
     2761 gggaaacggg actattttcc ttggcatacc aggagacaat aagcaggcta tgtcagaagc
     2821 attctatttc tatactttga gatgctctga agaggatttg agtgaagatc agaaaattgt
     2881 ctccaacagt cagacatcaa cagaagatcc tggggactcc actccctttg aagactcaga
     2941 ggaattttgt ttcagtgctg aagcaaccag ttttgatggt gacgatgaat ttgacaccta
     3001 caatgaagat gatgaagatg acgagtctgt aaccggctac tggataacat gttgccctac
     3061 ttgtgatgtt gacatcaata cctgggttcc gttctattca acggagctca ataaacccgc
     3121 catgatctat tgttctcatg gggatgggca ctgggtacat gcccagtgca tggatttgga
     3181 agaacgcaca ctcatccact tgtcagaagg aagcaacaag tattattgca atgaacatgt
     3241 acagatagca agagcattgc aaactcccaa aagaaacccc cccttacaaa aacctccaat
     3301 gaaatccctc cacaaaaaag gctctgggaa agtcttgact cctgccaaga aatcctaata
     3361 actcgagact agtatcgcga taattcactc ctcaggtgca ggctgcctat cagaaggtgg
     3421 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     3481 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     3541 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     3601 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     3661 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     3721 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     3781 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     3841 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     3901 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     3961 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4021 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4081 gctttccagt cgggaaacct gtcgtgccag cggatcgatc cgctgcatta atgaatcggc
     4141 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
     6121 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     6181 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     6241 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     6301 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     6361 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     6421 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     6481 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     6541 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     6601 ggggg
//
