LOCUS       sequence-223075-        8428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid suitable for experiments in environments where 
            there is no antibiotic selection present. Constitutive promoter pNeo
            expressing gusA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOPS0750
FEATURES             Location/Qualifiers
     source          1..8428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             295..2106
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVRYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      2120..2206
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2299..2393
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2503..3318
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            3469..3577
                     /note="incP origin of transfer"
     CDS             complement(3638..4786)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      5179..5710
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(8232..8318)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc
       61 cttcaactca gcaaaagttc gatttattca acaaagcgac gttgtgtctc aaaatctctg
      121 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
      181 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgagatc
      241 tatcgatgca tgccatggta cccgggagct cgaattcagg cttggaggat acgaatggtc
      301 cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt
      361 ctggatcgcg aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt acaagaaagc
      421 cgggcaattg ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat
      481 tatgcgggca acgtccggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag
      541 cgtatcgtgc tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag
      601 gaagtgatgg agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt
      661 attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact
      721 atcccgccgg gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat
      781 gatttcttta actatgccgg aatccatcgc agcgtaatgc tctacaccac gccgaacacc
      841 tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt
      901 gactggcagg tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag
      961 gtggttgcaa ctggacaagg cactagcggg actttgcaag tggtgaatcc gcacctctgg
     1021 caaccgggtg aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt
     1081 gatatctacc cgcttcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg
     1141 attaaccaca aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggacttgcgt
     1201 ggcaaaggat tcgataacgt gctgatggtg cacgaccacg cattaatgga ctggattggg
     1261 gccaactcct accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat
     1321 gaacatggca tcgtggtgat tgatgaaact gctgctgtcg gctttaacct ctctttaggc
     1381 attggtttcg aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc agtcaacggg
     1441 gaaactcagc aagcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac
     1501 ccaagcgtgg tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg
     1561 gaatatttcg cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc
     1621 tgcgtcaatg taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg
     1681 ctgtgcctga accgttatta cggatggtat gtccaaagcg gcgatttgga aacggcagag
     1741 aaggtactgg aaaaagaact tctggcctgg caggagaaac tgcatcagcc gattatcatc
     1801 accgaatacg gcgtggatac gttagccggg ctgcactcaa tgtacaccga catgtggagt
     1861 gaagagtatc agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc
     1921 gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc
     1981 gttggcggta acaagaaagg gatcttcact cgcgaccgca aaccgaagtc ggcggctttt
     2041 ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa aaccgcagca gggaggcaaa
     2101 caatgagctt cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2161 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     2221 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     2281 cagggaattc cgctcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     2341 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaggca
     2401 attgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     2461 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcagcgt
     2521 gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct gtatggctat
     2581 aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct gtatggcaaa
     2641 ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa cgatgtgacc
     2701 gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac cattaaacat
     2761 tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg caaaaccgcg
     2821 tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc gctggccgtg
     2881 tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag cgatcgtgtg
     2941 tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc gagcgatttt
     3001 gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca taaactgctg
     3061 ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa cctgattttc
     3121 gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc ggatcgttat
     3181 caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct gcaaaaacgt
     3241 ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt tcatctgatg
     3301 ctggatgaat ttttctaata attaattgaa ctacttttcc gctgcataac cctgcttcgg
     3361 ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg
     3421 ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg
     3481 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
     3541 ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggccgcg atgcaggtgg
     3601 ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt
     3661 catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc
     3721 cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg
     3781 tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg
     3841 ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa
     3901 acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt
     3961 ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg
     4021 tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc
     4081 ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct
     4141 cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt
     4201 cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga
     4261 ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc
     4321 gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg
     4381 aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggctt
     4441 cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc
     4501 ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agccgacgcg
     4561 aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc
     4621 gctcgatctt ggccgtagct tgctggacta tcgagccgac ggactggaag gtttcgcggg
     4681 gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt
     4741 cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg
     4801 ggattgcccc ggaattaatt ccccggatcg atccgtcgat cttgatcccc tgcgccatca
     4861 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga
     4921 gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta
     4981 tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt
     5041 ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt
     5101 ctacgtggct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg
     5161 ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg
     5221 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
     5281 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
     5341 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
     5401 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
     5461 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
     5521 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
     5581 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
     5641 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
     5701 gccggcggcc ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     5761 tcccccgcag aaaaaaagga tctcaagaag atcctttgat cttttctaca ttactgcatg
     5821 agcttgtgga agtgtgctga gcataaatga ttctcctagc tgttctttgg gtaagtacgc
     5881 catcaggacg ttgtgagtgg cgcgattttt agcggctgaa atcagccctt gagcctgtcg
     5941 gcaagtcgcg tcatgaggtc catgcgctca tgcaggatcg ccacgaccaa cgcgggttcg
     6001 cccgcacgcg gcaggcaaaa aacgtagtgg tgttcgcagc gggccatccg cagcgcggga
     6061 aagagttcgc tcatgtcctt aaacgggcct tcgccggcgg caagcctggc tatgccctgt
     6121 tccagcttag cgatatagcg gcgcacctgc gccgcgcccc actcccggcg cgtgtagcgg
     6181 atgatgccgc gtagatcggc ttcggcctca gccgtgagga tgtaggccgt caagcgcgat
     6241 ccccgctgag ttcttcatca agaatttcgc cgacgctctt ggtggacacc ttgccggcaa
     6301 gcccatcgtt gatgcggttc cccagcatgg ttttcagttc ctgccatgcc tgatcggcat
     6361 cagcgtcacc ggggaacaga cgttcgaggg cgtattgctt aatggtcttg ccctgcaagg
     6421 cggccagggc tttcaggctc tggtgctgct ggtccgtcat gtcgattgtc aggcggctca
     6481 ttggataacc tccataaaat acacgtaacc acattagcac atatgtgggc gtgaggctac
     6541 agcgcgaggc gcattaaggt cgggaaaatg cgctaggcgc atttaaattg cgtattgctg
     6601 taatgcgcca tgccggctag actaggccca aatgggtata cccaatttga ccaaggggga
     6661 cgcgatgagg gcggccaagc actaccgaca acttctatcc atcgacttca acatcgaggc
     6721 gctggccttc gtgcctggac ccgacggcac acgcggccgg cgcatccacg tcctggggcg
     6781 cgaggtccgc gaccggcccg gcctggtcga gtacctttcg ccggcgttcg gctcgcgggt
     6841 ggcgctggac ggctactgca aggccaattt cgatgcagtg ctgcacctgg cgtaccccga
     6901 tcatcagcaa tggggccacg catgaagcgc cgaagctacg ccatgctgcg cgccgctgcc
     6961 gcgctggccg tcctggtcgt tgcctcgccg gcatgggccg agctgcgcgg cgaggtcgtg
     7021 cgcatcatcg acggcgacac catcgacgtg ctggtagaca agcagccggt gcgcgtgcgc
     7081 ctggtggaca ttgacgcgcc ggaaaagcgg caagccttcg gcgaacgtgc gcgccaggcg
     7141 ctggccggca tggtgttccg ccggcacgtc ctggtcgacg agaaggacac cgaccgttac
     7201 ggccgcacgc tgggcaccgt ctgggtcaac atggagctgg ccagccggcc gccgcagccg
     7261 cgcaacgtca acgccgcgat ggttcaccag ggcatggcgt gggcctatcg cttccacggc
     7321 cgcgcggccg accctgaaat gctgcggctc gaacaggagg cgcgaggcaa gcgcgtcggc
     7381 ctctggtccg atccgcacgc cgtcgagccg tggaaatggc gacgcgagag caacaaccgg
     7441 agggacgaag gttgaaggtc gcccgcatct acctgcgcgc cagtacggac gagcagaatc
     7501 ttgaacgcca ggagagcctt gtagcggcca cgcgggccgc cgggtactac gtcgccggca
     7561 tctaccgcga gaaggcgtcc ggcgcacgcg ccgaccggcc cgagctgctg cgcatgatcg
     7621 cggacctgca acctggtgaa gtcgtcgttg cggagaagat cgaccgcatc agccgcttgc
     7681 cgttggccga ggccgagcgc ctggttgcgt cgatccgggc caaaggggcc aagctggccg
     7741 tgcctggcgt ggtggacctg tcggagctgg ccgccgaggc gaacggagtg gcgaaaatcg
     7801 ttctggaatc cgtccaggac atgcttttga agctcgcctt gcagatggcc cgcgacgact
     7861 acgaggatcg gcgcgagcgt caacgtcagg gtgtccagtt ggcgaaggcc gccggccgct
     7921 acaccggccg caaacgtgac gccggcatgc acgaccgcat catcacgctt cgctccggcg
     7981 gatcgagcat tgccaagacg gccaagctgg tcggatgcag cccgagccag gtcaaacgag
     8041 tgtgggcggc ctggaacgcg cagcagcaaa aataaagccg ggcagtgccc ggcttttctc
     8101 accttttcgc gtcccgcagg gccgctgcga gcgccctacc tagatcctcg ctttccccct
     8161 cggtgtagtc cggccagggc acgaagggcg cggatgcgaa cctgttgagc aggtcagatc
     8221 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     8281 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctgccgaa ttcggatccg
     8341 gagaggcctg aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag
     8401 ctttgttgta ggtggaccag ttggtgat
//
