LOCUS       sequence-223077-        4062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SC module. Beta-glucosidase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG083
FEATURES             Location/Qualifiers
     source          1..4062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1812
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVRYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     primer_bind     complement(1852..1870)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1938..2042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2264..3055
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3226..3814
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3715..3734
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3968..3985
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca
       61 ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa
      121 gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt
      181 cgtaattatg cgggcaacgt ccggtatcag cgcgaagtct ttataccgaa aggttgggca
      241 ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat
      301 aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg
      361 tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg
      421 cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac
      481 ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg
      541 aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg
      601 tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat
      661 caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac
      721 ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca
      781 gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag
      841 ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac
      901 ttgcgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg
      961 attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg
     1021 gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct
     1081 ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc
     1141 aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa
     1201 aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt
     1261 gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg
     1321 atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt
     1381 gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg
     1441 gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt
     1501 atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg
     1561 tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc
     1621 agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata
     1681 ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg
     1741 gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga
     1801 ggcaaacaat gagctttgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga
     1861 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1921 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2041 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2101 tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc
     2161 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2221 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat
     2281 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2341 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2401 cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2461 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2521 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2581 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2641 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     2701 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     2761 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     2821 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     2881 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     2941 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3001 ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc
     3061 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3121 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3181 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3241 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3301 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3361 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3421 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3481 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3541 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3601 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3661 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3721 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3781 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3841 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3901 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3961 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     4021 ccccgcgcgt tggccgattc attaatcact ctgtggtctc aa
//
