LOCUS       sequence-223084-        2654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector construction module. Endlinker/terminator module to 
            circularize plasmid following Part 6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG016
FEATURES             Location/Qualifiers
     source          1..2654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(410..496)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(644..661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(754..1342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(834..853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2527..2631)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccacact ctgtgcgtct cagagctcta gggcggcgga tttgtcctac
      421 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      481 cctttcgttt tatttgatgc ctgcctgaga cgcacgaagt gattaattaa ctggcctcat
      541 gggccttcac ttcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaac
      601 atggtcatag ctgtttcctt gcgtattggg cgctctccgc ttcctcgctc actgactcgc
      661 tgcgctcggt cgttcgggta aagcctgggg tgcctaatga gcaaaaggcc agcaaaaggc
      721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1501 ttggtctgac agttattatt tacccaccac tttggtaatt tcgcctttca cgtaatgcac
     1561 aaattcttcc agctgatccg cacggctggc cagacgatct tcttcctggc ccagatacgc
     1621 ctgacgcgct tccagaatca ccggctgata ctgcgccggc agacgttcca tggcccaatc
     1681 cgccgccaca tctttcggcg caattttgcc ggtcaccgcg ctataccaaa tacggctcag
     1741 ggtcagcacc acgttgcgtt catcacccgc ccaatccggc gggctgttcc acagggtcag
     1801 ggtttcgttc agcgcttcaa acaggtcctg ttccggcacc ggatcaaaca gttcttccgc
     1861 tgccggacca accagcgcca cgctatgttc acgcgctttg gtcagcagaa tggccagatc
     1921 gatatcaatg gtcgccggtt caaaaatgcc ggccagaata tcgttacgct gccattcgcc
     1981 aaactgcagc tcacgtttcg ccggataacg ccacggaata atgtcatcat gcaccacgat
     2041 ggtcacttcc accgcacgca gaatttcgct ttcacccggg ctcgcgctgg tttccagcag
     2101 atcgttaatc agcgcacgac gggtggtttc atccagacgc acggtcacgg tcaccagcag
     2161 atcaatatcg ctatgcggtt tcaggccgcc atccaccgcg ctgccataca gatgcacggc
     2221 cagcagggtc ggttccagat gacgttcaat cacgcccacc acttcagaca gctgggtgct
     2281 cacttccgca atcaccgctt cacgcataat gttcagtttg gttttcggac ggctgccctg
     2341 ctgggtcaca tcgttgctgc tccacagcat caggcaacgg cccaccgcat aacgcgcctg
     2401 ctgtttgctc gcacgcggca tgctctgcac gccaaaaaag caatcatagc acgccataaa
     2461 caccgccacc gcaccattac cgccgctacg ttcggtcagg gtacggctcc aattacggct
     2521 acgcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2581 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2641 ccgaaaagtg ccac
//
