LOCUS       sequence-223088-        8056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates 
            DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A
  TITLE     FOXP3 controls regulatory T cell function through cooperation with 
            NFAT.
  JOURNAL   Cell. 2006 Jul 28. 126(2):375-87.
  PUBMED    16873067
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Jul 28. 126(2):375-87."
COMMENT     Sequence Label: pRV.GFP FOXP3
FEATURES             Location/Qualifiers
     source          1..8056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             55..571
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    635..976
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1043..1459
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1389..1411
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1427..1443
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1484..1508
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1533..1542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1542..1571
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3075..3091)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3076..3092)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(3102..3126)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3158..3724
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3174..3726
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3318..3335)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3545..3564
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3738..3740,3741..3743,3744..4457)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3783..3804)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4044..4063)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4391..4412
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4504..5019
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5181..5197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5205..5211,5212..5229,5230..5235))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5250..5271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5388..5405)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5559..6147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5639..5658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6318..7109,7110..7178))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6941..6960
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7179..7283)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7351..7369
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7407..7429)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7529..7548
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7742..7764
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7892..7911
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
       61 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
      121 aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat
      181 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
      241 atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag
      301 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
      361 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
      421 ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtattc ccaataaagc
      481 ctcttgctgt ttgcatccga atcgtggact cgctgatcct tgggagggtc tcctcagatt
      541 gattgactgc ccacctcggg ggtctttcat ttggaggttc caccgagatt tggagacccc
      601 tgcccaggga ccaccgaccc ccccgccggg aggtaagctg gccagcggtc gtttcgtgtc
      661 tgtctctgtc tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc gtctgtacta
      721 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc
      781 cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg
      841 aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga
      901 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg
      961 cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1021 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     1081 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1141 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1201 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1261 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1321 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1381 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1441 cactccttct ctaggcgccg gaattagatc tctcgaggtc atcacaagtt tgtacaaaaa
     1501 agcaggcttc gaaggagata gaaccggtgg cggccgccat ggagcagaaa ctcatctctg
     1561 aagaggatct gagcccgggc ggatcccccg ggctgcagga attcggctcc tgggacaagg
     1621 acccgatgcc caaccccagg cctggcaagc cctcggcccc ttccttggcc cttggcccat
     1681 ccccaggagc ctcgcccagc tggagggctg cacccaaagc ctcagacctg ctgggggccc
     1741 ggggcccagg gggaaccttc cagggccgag atcttcgagg cggggcccat gcctcctctt
     1801 cttccttgaa ccccatgcca ccatcgcagc tgcagctgcc cacactgccc ctagtcatgg
     1861 tggcaccctc cggggcacgg ctgggcccct tgccccactt acaggcactc ctccaggaca
     1921 ggccacattt catgcaccag ctctcaacgg tggatgccca cgcccggacc cctgtgctgc
     1981 aggtgcaccc cctggagagc ccagccatga tcagcctcac accacccacc accgccactg
     2041 gggtcttctc cctcaaggcc cggcctggcc tcccacctgg gatcaacgtg gccagcctgg
     2101 aatgggtgtc cagggagccg gcactgctct gcaccttccc aaatcccagt gcacccagga
     2161 aggacagcac cctttcggct gtgccccaga gctcctaccc actgctggca aatggtgtct
     2221 gcaagtggcc cggatgtgag aaggtcttcg aagagccaga ggacttcctc aagcactgcc
     2281 aggcggacca tcttctggat gagaagggca gggcacaatg tctcctccag agagagatgg
     2341 tacagtctct ggagcagcag ctggtgctgg agaaggagaa gctgagtgcc atgcaggccc
     2401 acctggctgg gaaaatggca ctgaccaagg cttcatctgt ggcatcatcc gacaagggct
     2461 cctgctgcat cgtagctgct ggcagccaag gccctgtcgt cccagcctgg tctggccccc
     2521 gggaggcccc tgacagcctg tttgctgtcc ggaggcacct gtggggtagc catggaaaca
     2581 gcacattccc agagttcctc cacaacatgg actacttcaa gttccacaac atgcgacccc
     2641 ctttcaccta cgccacgctc atccgctggg ccatcctgga ggctccagag aagcagcgga
     2701 cactcaatga gatctaccac tggttcacac gcatgtttgc cttcttcaga aaccatcctg
     2761 ccacctggaa gaacgccatc cgccacaacc tgagtctgca caagtgcttt gtgcgggtgg
     2821 agagcgagaa gggggctgtg tggaccgtgg atgagctgga gttccgcaag aaacggagcc
     2881 agaggcccag caggtgttcc aaccctacac ctggcccctg acctcaagat caaggaaagg
     2941 aggatggacg aacaggggcc aaactggtgg gaggcagagg tggtgggggc agggatgata
     3001 ggccctggat gtgcccacag ggaccaagaa gtgaggtttc cactgtctta agccgaattc
     3061 gatatcaagc ttatcgatac cgtcgacctc gagatatcta gacccagctt tcttgtacaa
     3121 agtggtgatg acggtatcga taagcttgat atcgaattcc gccccccccc ctaacgttac
     3181 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     3241 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     3301 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     3361 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     3421 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     3481 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     3541 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     3601 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     3661 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     3721 ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3781 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtct ggcgagggcg
     3841 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3901 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     3961 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4021 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4081 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4141 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     4201 ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac atcgaggacg
     4261 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4321 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     4381 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4441 acgagctgta caagtaatga gataaaataa aagattttat ttagtctcca gaaaaagggg
     4501 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     4561 gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga acagagagac
     4621 agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     4681 aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat
     4741 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     4801 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     4861 acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtattccc
     4921 aataaagcct cttgctgttt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     4981 ctctgagtga ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt
     5041 ggagaacaac attctgaggg taggagtcga atattaagta atcctgactc aattagccac
     5101 tgttttgaat ccacatactc caatactcct gaaatagttc attatggaca gcgcagaaag
     5161 agctggggag aattgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5221 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5281 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5461 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5521 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5581 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5641 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5701 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5761 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5821 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5881 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5941 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6001 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6061 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6121 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6181 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6241 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6301 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6361 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6421 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6481 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6541 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6601 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6661 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6721 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6781 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6841 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6901 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6961 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7021 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7081 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7141 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7201 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7261 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     7321 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     7381 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     7441 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     7501 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     7561 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     7621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7681 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7741 tcccagtcac gacgttgtaa aacgacggcg caaggaatgg tgcatgcaag gagatggcgc
     7801 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     7861 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     7921 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc
     7981 aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc
     8041 agctgaagcc tataga
//
