LOCUS       sequence-223089-        2668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector construction module. Endlinker/terminator module to 
            circularize plasmid following Part 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG014
FEATURES             Location/Qualifiers
     source          1..2668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(417..503)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(658..675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(768..1356)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(848..867)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(2541..2645)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccatgag ctccactctg tgcgtctcac agatctaggg cggcggattt
      421 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
      481 gactgagcct ttcgttttat ttgatgcctg cctgagacgc acgaagtggg tacctcttaa
      541 ttaactggcc tcatgggcct tcacttcact gcccgctttc cagtcgggaa acctgtcgtg
      601 ccagctgcat taacatggtc atagctgttt ccttgcgtat tgggcgctct ccgcttcctc
      661 gctcactgac tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa
      721 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      781 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      841 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      901 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      961 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1021 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1081 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1141 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1201 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1261 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1321 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1381 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1441 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1501 atatatgagt aaacttggtc tgacagttat tatttaccca ccactttggt aatttcgcct
     1561 ttcacgtaat gcacaaattc ttccagctga tccgcacggc tggccagacg atcttcttcc
     1621 tggcccagat acgcctgacg cgcttccaga atcaccggct gatactgcgc cggcagacgt
     1681 tccatggccc aatccgccgc cacatctttc ggcgcaattt tgccggtcac cgcgctatac
     1741 caaatacggc tcagggtcag caccacgttg cgttcatcac ccgcccaatc cggcgggctg
     1801 ttccacaggg tcagggtttc gttcagcgct tcaaacaggt cctgttccgg caccggatca
     1861 aacagttctt ccgctgccgg accaaccagc gccacgctat gttcacgcgc tttggtcagc
     1921 agaatggcca gatcgatatc aatggtcgcc ggttcaaaaa tgccggccag aatatcgtta
     1981 cgctgccatt cgccaaactg cagctcacgt ttcgccggat aacgccacgg aataatgtca
     2041 tcatgcacca cgatggtcac ttccaccgca cgcagaattt cgctttcacc cgggctcgcg
     2101 ctggtttcca gcagatcgtt aatcagcgca cgacgggtgg tttcatccag acgcacggtc
     2161 acggtcacca gcagatcaat atcgctatgc ggtttcaggc cgccatccac cgcgctgcca
     2221 tacagatgca cggccagcag ggtcggttcc agatgacgtt caatcacgcc caccacttca
     2281 gacagctggg tgctcacttc cgcaatcacc gcttcacgca taatgttcag tttggttttc
     2341 ggacggctgc cctgctgggt cacatcgttg ctgctccaca gcatcaggca acggcccacc
     2401 gcataacgcg cctgctgttt gctcgcacgc ggcatgctct gcacgccaaa aaagcaatca
     2461 tagcacgcca taaacaccgc caccgcacca ttaccgccgc tacgttcggt cagggtacgg
     2521 ctccaattac ggctacgcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2581 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2641 ccgcgcacat ttccccgaaa agtgccac
//
