LOCUS       sequence-22319-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Blelloch R, Venere M, Yen J, Ramalho-Santos M
  TITLE     Generation of induced pluripotent stem cells in the absence of drug 
            selection.
  JOURNAL   Cell Stem Cell. 2007 Sep 13;1(3):245-7. doi: 
            10.1016/j.stem.2007.08.008. Epub 2007 Sep 6.
  PUBMED    18371358
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2007.08.008"; journalName: "Cell Stem Cell"; date: 
            "2007-09-13- 13"; volume: "1"; issue: "3"; pages: "245-7"
COMMENT     Sequence Label: pLOVE-Nmyc
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..113
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(151..192)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    245..269
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    886..897
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgta cctagtggaa
       61 ccggaaccct taaacatgta taacttcgta taatgtatgc tatacgaagt tattaggtcc
      121 ctcgacgaat tgtcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg
      181 gataggctta ccttcgaacc gcgggccctc tagactcgag cggccgccac tgtgctggat
      241 atcaaccact ttgtacaaga aagctgggtc tagatatctc gagtgcggcc gcgaattcca
      301 agtccgagcg tgttcgatct tctttagcaa ctgctgctgc ctcgcctgca gtttctcctt
      361 ttccagcagg agctggtgct cgttggcctg tagggcgtgc acgtactcgg tggccttttt
      421 caagatgacc accttggcgg ccttctcgtt cttcaccagc tcaggcacat ggtccctgag
      481 cgtcaggaag ctggagcgca ggtcgttccg gcgctgacgc tccaggatgt tgtggttgcg
      541 gcggcgctcg ctgtcctccg agtctgagtt tcgggggctc aggctcttcg cttttgctgg
      601 aacaacactt ttgagggggc gtggagaagc ctcgctcttg atctttttct gcgggggcgc
      661 gtcctcgctc tccacgtagg gtgagggtgc agcatagttg tgctgctgat ggatgggaac
      721 acagcgcttg aggatcagct cgccaggctg tgctcgcccc aggcccagag cggacgtctt
      781 gggacgcaca gtgatcgtga aagtggttac cgccttgttg ttagaggagg aacgtctctt
      841 ctctacggtg accacatcga tttcctcctc ttcatcttcc tcctcgtcat cctcatcatc
      901 tgagtcgctc aaggtatcct ctccgga
//
