LOCUS       sequence-223206-        1347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR template for dual guide RNA cloning, guide RNA scaffold for the 
            Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: pScaffold-H1 donor
FEATURES             Location/Qualifiers
     source          1..1347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..41
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     23..40
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(129..343)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(189..208)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        complement(350..425)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(495..514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(496..514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(521..538)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(521..537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(530..552)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     856..875
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 nnnnnannna ttangnnnag ctatttaggt gacactatag aatactcaag ctatgcatca
       61 agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt
      121 gggaaagagt ggtctcatac agaacttata agactcccaa atccaaagac atttcacgtt
      181 tatggtgatt tcccagaaca catagcgaca tgcaaatatt gcagggcgcc actcccctgt
      241 ccctcacagc catcttcctg ccagggcgca cgcgcgctgg gtgttcccgc ctagtgacac
      301 tgggcccgcg attccttgga gcgggttgat gacgtcagcg ttcaaaaaag caccgactcg
      361 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct
      421 aaaacaaggg cgaattctgc agatatccat cacactggcg gccgctcgag catgcatcta
      481 gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
      541 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      601 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      661 gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
      721 ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg
      781 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
      841 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
      901 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
      961 atgttctggg gaatataaat gtcaggcatg agattatcaa aaaggatctt cacctagatc
     1021 cttttcacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
     1081 tgggctatct ggacaaggga aaacgcaagc gcaaaaagaa agcaggtagc ttgcaagggg
     1141 cttanntggc cgatagctag acngggcggt tttatggann gcaagcgaac cggaattgcc
     1201 agctgggggn nccntctggg aaaggtggga aaccntgcaa gnnaaacggg agnntttctt
     1261 ggcccncaag gannnnaagg ggnccagggg aaaaancccc ttannaaaaa aanaaaaaga
     1321 atcntttccc annnttnnaa aaaaagg
//
