LOCUS       sequence-223208-        1578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: pSpCas9(BB)-T2A-HygR
FEATURES             Location/Qualifiers
     source          1..1578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             276..323
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             339..392
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcnaganagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
       61 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
      121 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
      181 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
      241 agacacggat cgacctgtct cagctgggag gcgacaaaag gccggcggcc acgaaaaagg
      301 ccggccaggc aaaaaagaaa aaggaattcg gcagtggaga gggcagagga agtctgctaa
      361 catgcggtga cgtcgaggag aatcctggcc caatgaaaaa gcctgaactc accgctacct
      421 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctccg
      481 agggcgaaga atctcgggct ttcagcttcg atgtgggagg gcgtggatat gtcctgcggg
      541 tgaatagctg cgccgatggt ttctacaaag atcgctatgt ttatcggcac tttgcatccg
      601 ccgctctccc tattcccgaa gtgcttgaca ttggggagtt cagcgagagc ctgacctatt
      661 gcatctcccg ccgtgcacag ggtgtcacct tgcaagacct gcctgaaacc gaactgcccg
      721 ctgttctcca gcccgtcgcc gaggccatgg atgccatcgc tgccgccgat cttagccaga
      781 ccagcgggtt cggcccattc ggacctcaag gaatcggtca atacactaca tggcgcgatt
      841 tcatctgcgc tattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
      901 tcagtgcctc cgtcgcccag gctctcgatg agctgatgct ttgggccgag gactgccccg
      961 aagtccggca cctcgtgcac gccgatttcg gctccaacaa tgtcctgacc gacaatggcc
     1021 gcataacagc cgtcattgac tggagcgagg ccatgttcgg ggattcccaa tacgaggtcg
     1081 ccaacatctt cttctggagg ccctggttgg cttgtatgga gcancagacc cgctacttcg
     1141 agcggaggca tcccgagctt gcaggatctc ctcggctccg ggcttatatg ctccgcattg
     1201 gtcttgacca actctatcaa aacttggttg acggcaattt ccatgaagca ccttgggctc
     1261 agggtccttg ccaaccaatc ctccnggtcc ggaaccggga nngngggggg taacaaaatc
     1321 ccccccaaaa atcctccccn nngggaccaa nnnnnnntgg naaangtccc cnnnnnaagg
     1381 naaaaaaang ctcccgctcn nnccnngngg gnaaaggttc gcntnntaaa ttcaaaagaa
     1441 gtgnnccgnn ataccccgca atggagccca ctgttnccnn ncgggtnngt tnccnancgc
     1501 gcttccctnn ntcntcaann ggnancgccc tgnnnnttnc cntnatnnng naaannggat
     1561 nnngnntnng aaattatt
//
