LOCUS       sequence-22321-e         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Blelloch R, Venere M, Yen J, Ramalho-Santos M
  TITLE     Generation of induced pluripotent stem cells in the absence of drug 
            selection.
  JOURNAL   Cell Stem Cell. 2007 Sep 13;1(3):245-7. doi: 
            10.1016/j.stem.2007.08.008. Epub 2007 Sep 6.
  PUBMED    18371358
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2007.08.008"; journalName: "Cell Stem Cell"; date: 
            "2007-09-13- 13"; volume: "1"; issue: "3"; pages: "245-7"
COMMENT     Sequence Label: pLOVE-Oct4
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    76..109
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(147..188)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    241..265
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtaatcca gaggttgatt atcgataagc ttgatatcga attgtaccta gtggaaccgg
       61 aacccttaaa catgtataac ttcgtataat gtatgctata cgaagttatt aggtccctcg
      121 acgaattgtc attactaacc ggtacgcgta gaatcgagac cgaggagagg gttagggata
      181 ggcttacctt cgaaccgcgg gccctctaga ctcgagcggc cgccactgtg ctggatatca
      241 accactttgt acaagaaagc tgggtctaga tatctcgagc cccaaagctc caggttctct
      301 tgtctacctc ccttgccttg gctcacagca tccccaggga gggctggtgc ctcagtttga
      361 atgcatggga gagcccagag cagtgacggg aacagaggga aaggcctcgc cctcaggaaa
      421 agggactgag tagagtgtgg tgaagtgggg gcttccatag cctggggtgc caaagtgggg
      481 acctgggggc agaggaaagg atacagcccc cccctgggaa accctgtagc ctcatactcc
      541 tctcgttggg aatactcaat acttgatctt ttgcccttct ggcgccggtt acagaaccat
      601 actcgaacca catccgtctc tagcccaagc
//
