LOCUS       sequence-22322-e         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Blelloch R, Venere M, Yen J, Ramalho-Santos M
  TITLE     Generation of induced pluripotent stem cells in the absence of drug 
            selection.
  JOURNAL   Cell Stem Cell. 2007 Sep 13;1(3):245-7. doi: 
            10.1016/j.stem.2007.08.008. Epub 2007 Sep 6.
  PUBMED    18371358
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2007.08.008"; journalName: "Cell Stem Cell"; date: 
            "2007-09-13- 13"; volume: "1"; issue: "3"; pages: "245-7"
COMMENT     Sequence Label: pLOVE-Sox2
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..113
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(151..192)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    245..269
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgta cctagtggaa
       61 ccggaaccct taaacatgta taacttcgta taatgtatgc tatacgaagt tattaggtcc
      121 ctcgacgaat tgtcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg
      181 gataggctta ccttcgaacc gcgggccctc tagactcgag cggccgccac tgtgctggat
      241 atcaaccact ttgtacaaga aagctgggtc tagatatctc gagcacatgt gcgacagggg
      301 cagtgtgccg tatttggccg tgccgggcac cgggccgctc tggtagtgct gggccatgtg
      361 cagtctactg ggcgcagcgg gctccggcac ctcggcgccg gggaggtaca tgctgatcat
      421 gtcccggagg tccccggcct ggcagggcgc cctggagtgg gaggaagagg taaccacggg
      481 ggggctggag ctggcctcgg acttgaccac agagcccatg gagcccagcg ccataccggg
      541 ggtgccctgc tgcgagtagg acatgctgta agtgggcgag ccgttcatgt aggtctgcga
      601 gctgggcatg gagttgtact gcagggcgct gac
//
