LOCUS       sequence-223233-        1403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPRa negative control. Catalytically inactive Cas9 from S. 
            pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the BFP CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 BFP-guide1
FEATURES             Location/Qualifiers
     source          1..1403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        145..163
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        215..233
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        320..531
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             548..595
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttnnagtttt aaattatntt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
       61 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgatg gcgtgcagtg
      121 cttcagcgtt ttagagctag gccaacatga ggatcaccca tgtctgcagg gcctagcaag
      181 ttaaaataag gctagtccgt tatcaacttg gccaacatga ggatcaccca tgtctgcagg
      241 gccaagtggc accgagtcgg tgcttttttt ggatcctagg tcttgaaagg agtgggaatt
      301 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
      361 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      421 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      481 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggcgtacggc
      541 caccatgaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaaggacaa
      601 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
      661 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      721 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      781 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      841 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      901 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
      961 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     1021 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     1081 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggaaaa
     1141 gctgttcatc cagctggtgc agacctacaa ccagctgttc caggaaaacc ccatcaacgc
     1201 caagcgcggg gaacgccaag gccatccttg cctgccaaaa tgaacaaaaa acaaaaggct
     1261 tggaaaattc tgaatgcccc acttgcccgg ggaagaaaaa aaatggcccg ggtccgccaa
     1321 cctgaatggc ccggaaccgg gggcctaaca ccccatttcc aaaggaacnt tnaactgggg
     1381 ccaaagatgg ccaactggct gtg
//
