LOCUS       sequence-223235-        1678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPRa negative control. Catalytically inactive Cas9 from S. 
            pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the EGFP CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 EGFP-guide1
FEATURES             Location/Qualifiers
     source          1..1678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        143..161
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        213..231
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        318..529
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             546..593
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnagtttta aattatgttt taaatggact atcatatgct taccgtaact tgaaagtatt
       61 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggaagt tcgagggcga
      121 cacccgtttt agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt
      181 aaaataaggc tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc
      241 caagtggcac cgagtcggtg ctttttttgg atcctaggtc ttgaaaggag tgggaattgg
      301 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      361 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      421 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      481 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca
      541 ccatgaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga
      601 agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg
      661 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
      721 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
      781 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
      841 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt
      901 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg
      961 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg
     1021 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt
     1081 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac atgggacaag
     1141 ctgttcatcc agctggtggc aaacctacaa ccagctgttc cagggaaacc ccatcaacgc
     1201 cagcggcgtg gaacccaagg ccatcctggc tgccaaaatg aacaaaaaca aaaggttggg
     1261 aaaactgatc cccccttggc ccgggaaaaa aaaaaaaggg cggtttcgga accctgatgg
     1321 ccctgagccg ggggcggaac cccaattcaa aaaaaatttt ccaccggggc agaaaggtca
     1381 caaaggggct tgaaaggagg acctttcaaa caaaactggg aacaacgttg ggcccaaaat
     1441 tgngnacaag tnncccactt gngttttggc cncaaaaaac tggnaaccca ctcctgtnng
     1501 aaagaacctt anagggagaa ccaaatataa acgggccctt cgggccttct taggtaagaa
     1561 taaaggaacc tcncggancg cctcgggaaa tctcttgggn nggngnacnt gggtaaaaaa
     1621 taggtattct tcaaaaaaaa gcatgggncc cggtttngga accccgaaga gaaaatta
//
