LOCUS       sequence-223237-        1845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPRa negative control. Catalytically inactive Cas9 from S. 
            pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 EGFP-guide2
FEATURES             Location/Qualifiers
     source          1..1845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        138..156
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        208..226
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        313..524
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             541..588
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttttaaatta tnttttaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
       61 ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagctggacg gcgacgtaaa
      121 gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat
      181 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
      241 ggcaccgagt cggtgctttt tttggatcct aggtcttgaa aggagtggga attggctccg
      301 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      361 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      421 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      481 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggcgtac ggccaccatg
      541 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagga caagaagtac
      601 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
      661 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
      721 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
      781 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
      841 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
      901 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
      961 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     1021 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     1081 ggccacttcc tgatcgaggg cgacctggaa ccccgaacaa cagcgaactg ggacaagctt
     1141 gttcatccag cttggtgcaa acctaacaac cagctggttc caaggaaaac cccatccacc
     1201 gccagcggcc ttgaacccca agggcattcc tggtctgcca aattggaaca aaaaacaaaa
     1261 cggttggaaa aatcgatacg cccaacttgc ccccgcaaga aaagaaaagg gcccttttcc
     1321 gcaacccgna tgtgccctga gnactgaggc ttgaccccca atttttnaaa aagaatttct
     1381 tcacctgggc caagaagatc caaacatgcg cgtgataaga gnggacacct aacanagaac
     1441 acactgnaaa acactctggt gggcccagta ntggcaacca aattggcaac aaattttttt
     1501 tggccgccac aaaatcaant cnacanacct nnccntctgt angagactcc cntagaaaga
     1561 ggaaanncgn naaatccaan ngcctcgggc cgntgngccc ttcnatatta aaaatggaaa
     1621 naaggccana cacccgcaaa aaaccgcgcc cgtggaacac tttcctccgg gnngnnggcc
     1681 cctgncgaaa tagaaaaaat ttnntacncn tncaaaaagg anaaaacccn cctcagtccn
     1741 tgngccaang caaaanatgt tatnnaccng ctcctccaga antggagaac acgggggctg
     1801 ccaanaaagg gcacagngtt cctgaacccc tgttctcaag ttgtc
//
