LOCUS       sequence-223241-         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2
            T...
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        43..118
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             422..434
                     /estimated_length=13
     gap             436..446
                     /estimated_length=11
     gap             463..472
                     /estimated_length=10
     gap             525..534
                     /estimated_length=10
     gap             594..614
                     /estimated_length=21
ORIGIN
        1 tntnnngnnn ggacgaaaca ccgggatcga aatccggagg aagttttaga gctagaaata
       61 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      121 ttttgaattc gctagctagg tcttgaaagg ggttggggtt gcgccttttc caaggcagcc
      181 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc ancggcgccg
      241 accctgggtc tcgcacattc ntcacgtccg ttcgcagcgt cncccggatc ttcnccgcta
      301 cccttgnggg ccccccggnn acgcttcctg ntccnncncn aannnngnaa ggntccttgc
      361 nnntcnnggn gngncggang ngacaaacnn aanccnnncn nctcactnnn ncnctcnnan
      421 annnnnnnnn nnnnannnnn nnnnnngcgc cnacnnnnan ggnnnnnnnn nnannnnnnn
      481 ngctcannnn nnnngntnan nnnnnngncn nnnnggnncn nnncnnnnnn nnnntgnnnn
      541 nnnncnnnnn nnnnannnnn cnnnnnnaan nnnnnangnn gntnnnngcn nnannnnnnn
      601 nnnnnnnnnn nnnnaancnt cnnagnnnnn nnna
//
