LOCUS       sequence-223247-        1277 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2
            T...
FEATURES             Location/Qualifiers
     source          1..1277
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        129..204
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        235..745
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     664..682
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      758..767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             794..979
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 annttgnagt tttaaattat gttttaaatg gactatcata tgcttaccgt aacttgaaag
       61 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga agggggatcg
      121 aaatccgggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      181 gaaaaagtgg caccgagtcg gtgctttttt gaattcgcta gctaggtctt gaaaggggtt
      241 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
      301 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
      361 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
      421 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
      481 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
      541 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
      601 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
      661 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
      721 ccgaatcacc gacctctctc cccaggaccg gtgatccgcc accatggatg ctaagtcact
      781 aactgcctgg tcccggacac tggtgacctt caaggatgta tttgtggact tcaccaggga
      841 ggagtggaag ctgctggaca ctgctcagca gatcgtgtac agaaatgtga tgctggagaa
      901 ctataagaac ctggtttcct tgggttatca gcttactaag ccagatgtga tcctccggtt
      961 ggagaaggga gaagagcccg gcggttccgg cgaaggtcga tggacaaaaa gtacagcatc
     1021 gtgctggcca tcgggacaaa ttctgtcggc tgggccgtga tcaccgacga gaacaggtgc
     1081 ccagcaagaa atcaaggggc tgggcaacac cgaccgggcn nnncttcaag aaaaacctga
     1141 ttggncgcct gctgttcgac cgtgaaaaat tgccaaggcc acccggttga aaaaaaccgc
     1201 cgaaaaaaga aacnccaatg gaaaaaccgg atttggattt ttgcaaagaa tttccaaacc
     1261 aaaaggncaa nttnnna
//
