LOCUS       sequence-223249-         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of GLIS3. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3
            TS...
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        122..197
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             463..474
                     /estimated_length=12
     gap             476..495
                     /estimated_length=20
     gap             582..593
                     /estimated_length=12
     gap             619..634
                     /estimated_length=16
ORIGIN
        1 gttttaaatt atnttttaaa atggactatn atatgcttac cgtaacttga aagtatttcg
       61 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggatgtgtg tgcggccgcg
      121 agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      181 tggcaccgag tcggtgcttt tttgaattcg ctagctaggt cttgaaaggg gttggggttg
      241 cgccttttcc aaggcagccc tgggtttgcg canggacgcg gctgctctgg gcgtggttcc
      301 gggaaannca ncggcgccna ccctgngtct cgcacattcn tcacgtccgt tcgcancgnc
      361 ncccggatct tcnccgctac ccttgngggn cccccggnna cnnttcnngn nncgccccta
      421 anncgnnaan gnnnnttgnn nntcnngnng ngnnnnannn gannnnnnnn nnnngnnnnn
      481 nnnnnnnnnn nnnnntnann nannaannca gnnnnnnnng nnagnnngnn naccgnnaan
      541 nntgngnnna anaannnann ntnnnnnnnn gnnnnnnnan annnnnnnnn nnntnnnnnn
      601 gnnnnnnnnn gnngngntnn nnnnnnnnnn nnnntnnnna nnnnnnnnan ta
//
