LOCUS       sequence-223251-        1442 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of GLIS3. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3
            TS...
FEATURES             Location/Qualifiers
     source          1..1442
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        123..198
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        229..739
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     658..676
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      752..761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             788..970
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEE"
ORIGIN
        1 anttttaaat tatnttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
       61 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgcagtgtg gacgcggctg
      121 cagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      181 gtggcaccga gtcggtgctt ttttgaattc gctagctagg tcttgaaagg ggttggggtt
      241 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
      301 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
      361 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
      421 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
      481 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
      541 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
      601 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
      661 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
      721 caccgacctc tctccccagg accggtgatc cgccaccatg gatgctaagt cactaactgc
      781 ctggtcccgg acactggtga ccttcaagga tgtatttgtg gacttcacca gggaggagtg
      841 gaagctgctg gacactgctc agcagatcgt gtacagaaat gtgatgctgg agaactataa
      901 gaacctggtt tccttgggtt atcagcttac taagccagat gtgatcctcc ggttggagaa
      961 gggagaagag ctcggcggtt ccggcggagg gtcgatggac aagaagtaca gcatcggcct
     1021 ggccatcggc accaactctg tcggctgggc cgtgatcacc gacgagtaca aggtgcccag
     1081 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
     1141 cgccctgctg ttcaacngcg aagaaacacc caagccaccg gctgaagaaa accgccaaag
     1201 aagatacccc aannggagaa ccggatcggc tttngcaaga attttcccna acggaagggn
     1261 agggtgaaaa aactttcttc ccgaanggaa aaacccttcc gtggggaaaa gataaaaaan
     1321 ccaaagggcc tccctttttc ggaaatttgg aaaagggcct ccccgaagaa cccccattta
     1381 accctgaaaa aaatggggga acccccataa agcccantcg gttattattt ggcccggccc
     1441 aa
//
