LOCUS       sequence-223253-        1385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of GLIS3. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3
            TS...
FEATURES             Location/Qualifiers
     source          1..1385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        121..196
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        227..737
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     656..674
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      750..759
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             786..971
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLDSLGY
                     QLTKPDVILRLEKXEEP"
ORIGIN
        1 ttttaaatta tnttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
       61 tttcttggct ttatatatct tgtggaaagg acgaaacacc ggcaattagc cagcatcccg
      121 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      181 ggcaccgagt cggtgctttt ttgaattcgc tagctaggtc ttgaaagggg ttggggttgc
      241 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
      301 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
      361 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
      421 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
      481 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
      541 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
      601 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
      661 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
      721 ccgacctctc tccccaggac cggtgatccg ccaccatgga tgctaagtca ctaactgcct
      781 ggtcccggac actggtgacc ttcaaggatg tatttgtgga cttcaccagg gaggagtgga
      841 agctgctgga cactgctcag cagatcgtgt acagaaatgt gatgctggag aactataaga
      901 acctggattc cttgggttat cagcttacta agccagatgt gatcctccgg ttggagaagn
      961 nagaagagcc cggtggttac cggtggaggt tcgatggaca agaagtacgg aatcggactg
     1021 gnnancggca ccaactctga cggctgggcc nngatcacca acgagtacaa ggtgcccatc
     1081 aagaaattca aggtgctggn caacaccgac cggnnaaggt tcaagaagaa cctgatcggg
     1141 nncctgctgt tcgacagcgg aaaaaaagcc gaggcccccc ggttgaanga aaccgcccga
     1201 agaagaatcc cccaacggaa aaacccggat ttgctttttg caaaaatctt taccaatgaa
     1261 ttggccaagg gggacaaacc ttttttccat aaatggaaaa atcctttctg ggggaaaaaa
     1321 ttaaaatccc aaacggatcc cttttttcac aattttggan gaanggtccn nnctnnaatt
     1381 nnacc
//
