LOCUS       sequence-223257-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D
            T...
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        33..108
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             263..274
                     /estimated_length=12
     gap             346..358
                     /estimated_length=13
     gap             390..399
                     /estimated_length=10
     gap             467..483
                     /estimated_length=17
     gap             491..503
                     /estimated_length=13
     gap             553..573
                     /estimated_length=21
     gap             594..609
                     /estimated_length=16
     gap             632..645
                     /estimated_length=14
     gap             670..680
                     /estimated_length=11
ORIGIN
        1 gacgaaacac cggcaaagga gccggcgcgc gggttttaga gctagaaata gcaagttaaa
       61 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgaattc
      121 gctagctagg tcttgaaagg ggttggggnt gngccttttc caggnagccc tgggtttgnn
      181 nnngnnnncn gntgctctgg nnnngnncnc nngaaacgnn nngnnnccna ccctgngnnn
      241 cgnncnntcn ncnnnnncnn ncnnnnnnnn nnnngancnt cnncgnnacn cnngnnnnnc
      301 accggnnann nnncngnnnn gncnctaant nctgaangtn nnnngnnnnn nnnnnnnngn
      361 nngannnnnn anannnnnnn tnnnngnntn nnnnnnnnna ttannaancn nnnnnncnnn
      421 gcnntttgnn annnttcnnn nnnnncatct nnnnnnnnan nnnnntnnnn nnnnnnnnnn
      481 nnnanannta nnnnnnnnnn nnncnntacn nnnncnnnnn nnnntctgnn nnnntcagag
      541 ntnnnnnnnn ncnnnnnnnn nnnnnnnnnn nnntnttnnn tncngncnnn nngnnnnnnn
      601 nnnnnnnnna nnnnnnnnnt nnnntnntnn tnnnnnnnnn nnnnntannc tnaannannn
      661 nnannnnngn nnnnnnnnnn tnt
//
