LOCUS       sequence-223261-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D
            T...
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        47..122
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             579..594
                     /estimated_length=16
     gap             602..624
                     /estimated_length=23
     gap             630..647
                     /estimated_length=18
     gap             652..662
                     /estimated_length=11
     gap             670..681
                     /estimated_length=12
     gap             704..716
                     /estimated_length=13
     gap             757..768
                     /estimated_length=12
     gap             774..792
                     /estimated_length=19
     gap             820..831
                     /estimated_length=12
     gap             873..893
                     /estimated_length=21
ORIGIN
        1 tntcttgtgg aaaggacgaa acaccgagcg aaagaggaaa ctgcaggttt tagagctaga
       61 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      121 gcttttttga attcgctagc taggtcttga aaggggttgg ggttgcgcct tttccaaggc
      181 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
      241 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
      301 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
      361 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
      421 gcagacggac agcgccnggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
      481 agcggctgct cagcagggcg cgccgagagc ancggccggg aaggggcggt gcgggaggnn
      541 nnnngnggnn nggnagngng nnnannnnnn gnnnnnaann nnnnnnnnnn nnnngnaann
      601 tnnnnnnnnn nnnnnnnnnn nnnngnnnan nnnnnnnnnn nnnnnnngnn gnnnnnnnnn
      661 nnanngangn nnnnnnnnnn ngnnagnnnn nnnnnaannn nngnnnnnnn nnnnnnatga
      721 nngtnnnnna anatnnnngn nnnnnnatct nnnnngnnnn nnnnnnnntn ntgnnnnnnn
      781 nnnnnnnnnn nnannnnnnc nnnnannnnn annnnnnnan nnnnnnnnnn ngnnnnnncn
      841 ncntnncnnn nnngagnnan nnnnnnnann ngnnnnnnnn nnnnnnnnnn nnngnannnn
      901 gnnnnntnnn nnnnngna
//
