LOCUS       sequence-223263-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D
            T...
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        41..116
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             575..588
                     /estimated_length=14
     gap             629..638
                     /estimated_length=10
     gap             661..672
                     /estimated_length=12
     gap             719..731
                     /estimated_length=13
     gap             743..759
                     /estimated_length=17
     gap             777..800
                     /estimated_length=24
ORIGIN
        1 tnngnnngga cgaaacaccg ggcggcggcg agagcgaaag gttttagagc tagaaatagc
       61 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
      121 ttgaattcgc tagctaggtc ttgaaagggg ttggggttgc gccttttcca aggcagccct
      181 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
      241 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
      301 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
      361 ttcgcggcgn gncggacgng acaaacngaa nccgcacgtc tcactannac ccnnnnnnac
      421 ngacagcnnc nnngannnnn gnnnnnnnnc cnaccgnnat ggnntgngnn cnanngnggn
      481 tgctcagcan gnnncgccna nnncancgnn cngnaannnn cggngcgnnn gccnannnnn
      541 nncnngnnnn nnnntnantn nnnnannnnn anacnnnnnn nnnnnnnnan nttnnnnnnn
      601 nccnnnnngn nnnnnnnngn nannnacann nnnnnnnngt tnnnnnntnn cnnnatgnnc
      661 nnnnnnnnnn nnaannanna ntttttttnt nnatagtttt gnnnnnannn nncnnnntnn
      721 nnnnnnnnnn ngnnnnnnnt aannnnnnnn nnnnnnnnnc nnnnnnnann ncnnncnnnn
      781 nnnnnnnnnn nnnnnnnnnn gtcnannnnn anngnnnngg gnannnnnnn nntanccnnn
      841 ntnnnannnn nnnnntnncn nnnnncnaaa aannnangan ntnnnnanaa cnannnntnn
      901 tnnnnnnnnn tnnnnnna
//
