LOCUS       sequence-223265-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of OPTN. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN
            TSS...
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        38..113
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             555..564
                     /estimated_length=10
     gap             571..582
                     /estimated_length=12
     gap             594..607
                     /estimated_length=14
     gap             609..621
                     /estimated_length=13
     gap             637..647
                     /estimated_length=11
     gap             649..658
                     /estimated_length=10
     gap             665..682
                     /estimated_length=18
     gap             710..722
                     /estimated_length=13
     gap             752..762
                     /estimated_length=11
     gap             788..805
                     /estimated_length=18
     gap             824..838
                     /estimated_length=15
ORIGIN
        1 gnnnggacga aacaccggcg gagggggcag ccgggacgtt ttagagctag aaatagcaag
       61 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg
      121 aattcgctag ctaggtcttg aaaggggttg gggttgcgcc ttttccaagg cagccctggg
      181 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
      241 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
      301 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
      361 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
      421 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tancggctgc
      481 tcagcagggc gcgcnnannn cnncggccgg gaaggggcnn ngnnnnnnnn ggnnngtgcn
      541 gcggnaannn nnnannnnnn nnnncaanaa nnnnnnnnnn nnaaanagna nngnnnnnnn
      601 nnnnnnngnn nnnnnnnnnn nannncnnnn nannnannnn nnnnnnnann nnnnnnnngn
      661 nangnnnnnn nnnnnnnnnn nnannnggan nannnnnnng ggnnnnnnan nnnnnnnnnn
      721 nnannnnnat nngnntnann nngaannnnn gnnnnnnnnn nnagnnntnn aannnnntnn
      781 nnnnnngnnn nnnnnnnnnn nnnnncnnnn gtgnnnnnnn nngnnnnnnn nnnnnnnngn
      841 nngnncnann nnngcnnnnn nntcnnnnnn agnnnnnnnn nat
//
