LOCUS       sequence-223267-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of OPTN. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN
            TSS...
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        54..129
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             596..607
                     /estimated_length=12
     gap             731..744
                     /estimated_length=14
     gap             750..759
                     /estimated_length=10
ORIGIN
        1 ttttnnntnn nttgnggaaa ggacgaaaca ccgggtcagc gtcccatccc ggtgttttag
       61 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      121 agtcggtgct tttttgaatt cgctagctag gtcttgaaag gggttggggt tgcgcctttt
      181 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
      241 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
      301 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
      361 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
      421 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
      481 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
      541 ggaggcgggg tgtgnnncgg nagngngggn nnnnnnccnn nnnnnncnnn ngnncnnnnn
      601 nnnnnnngcc anngnannnn nnnncnncng nagnnnnnnn annngnnnga nnnnngnnnn
      661 nnnnnannng nancaggtna nnanncaana ngnnnnnnna nnnncnnnnn gnaaggnnac
      721 nngnannana nnnnnnnnnn nnnnanntan nnnnnnnnnc tacnnnacnn nnnccgangn
      781 a
//
