LOCUS       sequence-223269-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of OPTN. KRAB domain and catalytically 
            inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN
            TSS...
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        37..112
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             184..195
                     /estimated_length=12
     gap             203..213
                     /estimated_length=11
     gap             370..381
                     /estimated_length=12
     gap             385..394
                     /estimated_length=10
     gap             460..469
                     /estimated_length=10
     gap             494..511
                     /estimated_length=18
     gap             580..595
                     /estimated_length=16
     gap             668..678
                     /estimated_length=11
     gap             701..713
                     /estimated_length=13
ORIGIN
        1 aaaggacgaa acaccggaga gaactcccga ccgggagttt tagagctaga aatagcaagt
       61 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttga
      121 attcgctagc taggtcttga aaggggttgg ggntgcnccn nntccnaggn anncctgnnn
      181 ntgnnnnnnn nnnnngntgn tannnnnnnn nnnccggnnn nnnnnntnnn nnannnngng
      241 nctngnannn nnnncnnnnn gnnnnnnnnt cnccnngntc tgccnnnnta nccnngnnnn
      301 nnnngnnnnn angttnnngn nnnnnncnna nnncngnaan nttngatgnn nttggnggtg
      361 ngannnnngn nnnnnnnnnn natcnnnnnn nnnnannngn ncngcgnnnn annnnnnncn
      421 ncnannannn nngnnnnaan gcnncnnnnn nnaggagctn nnnnnnnnnt nngcccancc
      481 ngnnnnancn ngannnnnnn nnnnnnnnnn ncnannagnn ngtgtgcnnn anntncnatg
      541 nnnnnncncc ntcnnnnnnn tnnccctnnn ntnnannatn nnnnnnnnnn nnnnncnnnn
      601 cctnnngnnn nnncnnnncn nnnnnngnnn ngnnnnnann nnnncanccn nnagcnngan
      661 nncgtgannn nnnnnnnntn nncantnnnc ccntgcccga nnnnnnnnnn nnnancnnan
      721 cnncnnncnn nnnngnnnnn nnngnnnana nccncgncc
//
