LOCUS       sequence-22327-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     Buchler lab plasmids
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p405MET25
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        68..417
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     418..434
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     418..434
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(468..484)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(469..485)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      487..734
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(503..521)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(751..770)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(752..770)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atttaccctc actaaaggga acaaagctgg agctccggat gcaagggttc gaatccctta
       61 gctctcatta ttttttgctt tttctcttga ggtcacatga tcgcaaaatg gcaaatggca
      121 cgtgaagctg tcgatattgg ggaactgtgg tggttggcaa atgactaatt aagttagtca
      181 aggcgccatc ctcatgaaaa ctgtgtaaca taataaccga agtgtcgaaa aggtggcacc
      241 ttgtccaatt gaacacgctc gatgaaaaaa ataagatata tataaggtta agtaaagcgt
      301 ctgttagaaa ggaagttttt cctttttctt gctctcttgt cttttcatct actatttcct
      361 tcgtgtaata cagggtcgtc agatacatag atacaattct attaccccca tccatactct
      421 agaactagtg gatcccccgg gctgcaggaa ttcgatatca agcttatcga taccgtcgac
      481 ctcgagtcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc
      541 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag
      601 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac
      661 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaangttttg ggacgctcga
      721 aggctttaat ttgcggccgg tacccattcg ccctatagtg agtcgtatta cgcgcgctca
      781 ctgggcgtcg tttttacacg tcgtgactgg g
//
