LOCUS       sequence-223273-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 TCF7L2 TSS-guide2
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        139..157
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        209..227
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        314..524
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     gap             665..675
                     /estimated_length=11
     gap             699..712
                     /estimated_length=14
ORIGIN
        1 gttttaantt annttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
       61 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggaagccgg ggggagacgg
      121 agttttagag ctaggccaac atgaggatca cccatgtctg cagggcctag caagttaaaa
      181 taaggctagt ccgttatcaa cttggccaac atgaggatca cccatgtctg cagggccaag
      241 tggcaccgag tcggtgcttt ttttggatcc taggtcttga aaggagtggg aattggctcc
      301 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      361 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      421 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcnntantc
      481 gccgtgaacg ttctttttcg caacgggttt gccnccanaa cacangcgta cggccaccat
      541 gananggccg gcggccncna naaangncgg ncannnanaa aagaaanagg annnnnnnna
      601 cngcntcnnn ntggncatnn gnncnnactc tgnggnntgg gccgngatnn ccgatannan
      661 nnngnnnnnn nnnnnganan tcnangngnn ngnnnanann nnnnnnnnnn nnancnannn
      721 nannntgatn ngnannnnng
//
