LOCUS       sequence-223275-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 TCF7L2 TSS-guide3
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        137..155
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        207..225
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        312..523
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             540..587
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttttaaatta tgttttaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
       61 ttcttggctt tatatatctt gtggaaagga cgaaacaccg cggaggaacg gagtagtctg
      121 ttttagagct aggccaacat gaggatcacc catgtctgca gggcctagca agttaaaata
      181 aggctagtcc gttatcaact tggccaacat gaggatcacc catgtctgca gggccaagtg
      241 gcaccgagtc ggtgcttttt ttggatccta ggtcttgaaa ggagtgggaa ttggctccgg
      301 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      361 cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      421 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      481 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggcgtacg gccaccatga
      541 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggac aagaagtaca
      601 gcaacggcct ggccatcggc accaactctg tgagctgggc cgtgatcacc gacgagtaca
      661 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccgngcacag catcaaaaag
      721 aacctgatcg gagcactgct gttcgacagc ggctaaacag ccgaggccac cnggctgata
      781 tagaaccgcn gannataata caccaganng aaagaaccga aactgctatc tgcgaaagat
      841 cttatnnacg agatgnccaa ngatggaaga cagctatctt acanangact gnnaagagtc
      901 ccttcctgga tggaaagagg anaaagannn cacgaaacga nnactccaat catcg
//
