LOCUS       sequence-223277-        1617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 TCF7L2 TSS-guide4
FEATURES             Location/Qualifiers
     source          1..1617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        141..159
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        211..229
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        316..527
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             544..591
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 anttttaaaa ttatntttta aaatggacta tcatatgctt accgtaactt gaaagtattt
       61 cgatttcttg gctttatata tcttgtggaa aggacgaaac accggggagc ggagattatt
      121 gacgttttag agctaggcca acatgaggat cacccatgtc tgcagggcct agcaagttaa
      181 aataaggcta gtccgttatc aacttggcca acatgaggat cacccatgtc tgcagggcca
      241 agtggcaccg agtcggtgct ttttttggat cctaggtctt gaaaggagtg ggaattggct
      301 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      361 gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      421 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      481 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggcg tacggccacc
      541 atgaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa ggacaagaag
      601 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
      661 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
      721 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
      781 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
      841 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
      901 ttcctgggtg aaagaggata agaagcacga acgggcaccc catcttncnn caacatcatg
      961 gaacgaagng ggcctaccac gannaattac gccacnntct accnncctgg aaaagagact
     1021 ggtgggacag cacccgacaa aggccgaccn tgcgggctga tctaatctag gcccctgggc
     1081 ccannatgga tnnannttcg cgnnccnnac tttcctggaa cngaagagca gaccntgaga
     1141 cccccgtacc annntccnga catgganaag agctggttcc atccaagctg ggtggacgaa
     1201 ccnnancgag canncnttgt nncagaagaa annnnaccac nnccaagctc ccaagctggc
     1261 tgttgaannc tcannnnnnn atccggnnnc catgcangaa anttagtagg caagaaacag
     1321 aacangcgat tggnnaaaga tctttagtcn agcccnnaac nnnacacccn ggccnnaaga
     1381 aaaagaaaaa aagtggaccc agtttttgga aggaagacca tgaatnacaa ccntnnnaaa
     1441 gcctnnggga nnnnaagcca ggccaaanna ttctaaaggg aannaaaagn tatnnaccgg
     1501 gaccnatggc anaggnnngn nnacccgcaa angnnattgg caatgggctt agganngaga
     1561 cnaannctcc canagganng naaacaaant cgnnttaaan nnccaaggct tggnccc
//
