LOCUS       sequence-223281-        1422 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of GLIS3. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 GLIS3 TSS-guide2
FEATURES             Location/Qualifiers
     source          1..1422
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        139..157
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        209..227
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        314..525
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             542..589
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1100..1111
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttttnaatta tnttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
       61 tttcttggct ttatatatct tgtggaaagg acgaaacacc gcactaggct ggccaattcg
      121 agttttagag ctaggccaac atgaggatca cccatgtctg cagggcctag caagttaaaa
      181 taaggctagt ccgttatcaa cttggccaac atgaggatca cccatgtctg cagggccaag
      241 tggcaccgag tcggtgcttt ttttggatcc taggtcttga aaggagtggg aattggctcc
      301 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      361 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      421 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      481 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggcgta cggccaccat
      541 gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg acaagaagta
      601 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
      661 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
      721 gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa
      781 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
      841 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
      901 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtgggacg
      961 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     1021 gcaccgacaa ggccgacctg ccggctgatc tatctgggcc tgggcccaca tnatcaagtt
     1081 ccgggggcca ctttcctgga tcgagggncg acctgaacct ccgaccaaca nnnaacgggg
     1141 gaacaagctg ttncttccag nngggtggca gacctanaac caggctggtt ccaaggaaaa
     1201 acccccatca aacgccaggc gggcggggga accccaaggg ccatccctgt ttgcccaaaa
     1261 tggagacaaa aanaaaaggg tttgggaaaa atcttgaatn ccccagtttg ccccggggaa
     1321 gaaaaaaaaa aaagggcccg tttccggaaa anctggaatt ggccctggaa aaccngggcc
     1381 tggaaacccc ccaatttttc agaaaaaaat ttntnaatgg gg
//
