LOCUS       sequence-223283-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of GLIS3. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 GLIS3 TSS-guide3
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..16
                     /estimated_length=15
     primer_bind     40..59
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        155..173
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        225..243
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     gap             512..523
                     /estimated_length=12
     gap             544..556
                     /estimated_length=13
     gap             577..587
                     /estimated_length=11
     gap             608..618
                     /estimated_length=11
     gap             665..684
                     /estimated_length=20
ORIGIN
        1 annnnnnnnn nnnnnngntt nnnattannt tttaaaatgg actatcatat gcttaccgta
       61 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgtg
      121 tgtgcggccg cgagggcgtt ttanagctag gccaacatga ggatcaccca tgtctgcagg
      181 gcctancaag ttaaaataan gctantccgt tatcaacttg gccaacatga ggatcaccca
      241 tgtctgcagg gncnagtggc accgagtcgg tgcttttttt ggatcctang tcttgagngg
      301 antggnaatt gnntccnnng ncnnncnggn nnnnancgcn cnnnnnccnc tntcnncnag
      361 aagtcgnnnn gnnnnnacct acncatattc ncctgaatca ctgatttatt gggannaact
      421 gnnaaagtga tntcnagnac tggntccgcc ttnttcncga nantgnnnnn nnannnnnta
      481 tannggnnnt ntnnnnnngn nnntncannt tnnnnnnnnn nnngncnnnn nncnnnnnan
      541 anannnnnnn nnnnnnagga nnnnnnnntn nnnnnannnn nnnnnnncnt nnnanannnn
      601 naannnannn nnnnnnnngn nnnnnnatnn ncnnnnnnnn ngngnnnnna nnnanncngn
      661 ngntnnnnnn nnnnnnnnnn nnnntnna
//
