LOCUS       sequence-223285-        1204 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of GLIS3. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 GLIS3 TSS-guide4
FEATURES             Location/Qualifiers
     source          1..1204
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        143..161
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        213..231
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        318..529
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             546..593
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttgnagtttt aaattatgtt ttaaatggac tatcatatgc ttaccgtaac ttgaaagtat
       61 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgtgtg tgtgaatgcg
      121 tcaaggtttt agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt
      181 aaaataaggc tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc
      241 caagtggcac cgagtcggtg ctttttttgg atcctaggtc ttgaaaggag tgggaattgg
      301 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      361 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      421 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      481 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca
      541 ccatgaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga
      601 agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg
      661 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
      721 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
      781 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
      841 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacata ctgtaataat
      901 ccttcctggt ggaagaggan aagaagcacg agcggcactc catcttcggc aacatcgtgg
      961 acgaggtggc ctaccacgaa annacgccac catctaccac ctgaagaaag aaactggtgg
     1021 ganngcaacg aacaatgcta acctgcgggt gatcaatnng ggccctggcg cacatgaatc
     1081 naanttccgg ggccacattc ctgaatcaag gncgtacgtg aacccnanng aannngagag
     1141 ctgaangaan gttgttgcat ccagaatggt nnnagancta aannncgact tgattcaatg
     1201 tnaa
//
