LOCUS       sequence-223289-        1631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 CAMK1D TSS-guide2
FEATURES             Location/Qualifiers
     source          1..1631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        144..162
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        214..232
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        319..530
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             547..594
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1117..1128
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttgnagtttt aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
       61 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgccc gaaggcggcg
      121 cgctgggttt tagagctagg ccaacatgag gatcacccat gtctgcaggg cctagcaagt
      181 taaaataagg ctagtccgtt atcaacttgg ccaacatgag gatcacccat gtctgcaggg
      241 ccaagtggca ccgagtcggt gctttttttg gatcctaggt cttgaaagga gtgggaattg
      301 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      361 gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      421 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcaa
      481 taatcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gcgtacggcc
      541 accatgaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggacaag
      601 aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac
      661 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
      721 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
      781 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
      841 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
      901 tccttcctgg tggaagagga taaaaagcac gagcggcacc ccatcttccg caacatcgtg
      961 gaacgaggtg gcactaccac gagaaggtac ccccaccatc taccacctgg aaaaagaaaa
     1021 ctggtgggac agcacccgac aaggccgaac ctgccggctg gatctatctg ggccctggcc
     1081 ccacatgaat caaggttccg ggggccactt tcctgaatcg aagggcgaac ctgaaccccg
     1141 aaaaacaggc aacgtgtaac aagcttgttc ctcccagctg gggggaagac tttacaacca
     1201 gctggtttca aaggaaaacc cccttcaacc cccacgcgga ctggggaacc ccaaggngcc
     1261 atccttgtct cgcctaaact ggtaggcaaa agccataaac ggctngtagt aaatccctga
     1321 actggcccaa ctttgccctt gggcgaagaa ggaaaaataa agggcccggt acncggacta
     1381 acccgttaat tggccccntg aaaccgtgng nnncggtaaa ccccccaann ttttcaagga
     1441 aagaaacttt tgtgtaacct ttgggctnaa aagtaattgt ccaanttgta gtttnttgta
     1501 naatccanag ttccccttta ncatttggca aaactttcgg gattacnctt tctttgggct
     1561 ntgcacatta tcggttttnc cagttagggn ttgggcccaa tagaggtttt taactgcngg
     1621 cctctgaacg c
//
