LOCUS       sequence-22329-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  TITLE     Buchler lab plasmids
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p405GAL1
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..481
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    40..157
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     452..475
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     496..512
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     496..512
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(546..562)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(547..563)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(581..599)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tttaccctca ctaaagggac aaaagctgga gctctagtac ggattagaag ccgccgagcg
       61 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt
      121 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact
      181 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat
      241 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct
      301 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa
      361 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca
      421 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga
      481 gaaaaaaccc cggattctag aactagtgga tcccccgggc tgcaggaatt cgatatcaag
      541 cttatcgata ccgtcgacct cgagtcatgt aattagttat gtcacgctta cattcacgcc
      601 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc
      661 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc
      721 ttttttttct gtacagacgc gtgtacgcat ggtacattat act
//
