LOCUS       sequence-223295-         533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from
            S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 OPTN TSS-guide1
FEATURES             Location/Qualifiers
     source          1..533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        123..141
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        193..211
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 aaaatnggac tatnatannc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
       61 tatcttgtgg aaaggacgaa acaccggagg ggcggggcct tggtcgtttt agagctaggc
      121 caacatgagg atcacccatg tctgcagggc ctagcaagtt aaaataaggc tagtccgtta
      181 tcaacttggc caacatgagg atcacccatg tctgcagggc caagtggcac cgagtcggtg
      241 ctttttttgg atcctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
      301 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
      361 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      421 tttttcccga gggnggggga gaaccgtata tnantgcant anncnccntg aacgttcttt
      481 ttcgcaacgg gtttgccgcc ngaannnnnn cgtacggcca ccatgannng gnc
//
