LOCUS       sequence-223299-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from
            S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 OPTN TSS-guide3
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        142..160
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        212..230
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        317..528
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             545..592
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnnagtttt aattatgttt taaatggact atcatatgct taccgtaact tgaaagtatt
       61 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggatgg gcggggtatg
      121 ggatgtttta gagctaggcc aacatgagga tcacccatgt ctgcagggcc tagcaagtta
      181 aaataaggct agtccgttat caacttggcc aacatgagga tcacccatgt ctgcagggcc
      241 aagtggcacc gagtcggtgc tttttttgga tcctaggtct tgaaaggagt gggaattggc
      301 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
      361 ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      421 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      481 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggc gtacggccac
      541 catgaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa aggacaagaa
      601 gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga
      661 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc gcagcatcaa
      721 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct
      781 gaagagaacc gccagaacaa gatacaccag acggtaagaa ccgggatctg gctatctggc
      841 agcagatctt tcagcaacga agatggccca acggtggaac gaacancttc ctttccacna
      901 gaaccggcaa gaagtccttt cctggtggga atgagggata agtaaatcaa cgattcggca
      961 tccccattct ttccgcgaac cgtcggagga acg
//
