LOCUS       sequence-22330-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     Buchler lab plasmids
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p405GALL
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     427..450
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     471..487
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     471..487
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(521..537)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(522..538)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(556..574)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg gagctcgtga cagccctccg aaggaagact
       61 ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc
      121 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa
      181 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag
      241 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg
      301 atttttgatc tattaacgga tatataaatg caaaaactgc ataaccactt taactaatac
      361 tttcaacatt ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa
      421 attgttaata tacctctata ctttaacgtc aaggagaaaa aaccccggat tctagaacta
      481 gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagt
      541 catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga
      601 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt
      661 agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta
      721 cgcatgtaac attatactga aaacct
//
