LOCUS       sequence-223301-        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from
            S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 OPTN TSS-guide4
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        145..163
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        215..233
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        320..531
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 anntnnngtt ttanattatg ttttaaatgg actatcatat gcttaccgta acttgaaagt
       61 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggc tgggacccgg
      121 gtgaacagtt ttagagctag gccaacatga ggatcaccca tgtctgcagg gcctagcaag
      181 ttaaaataag gctagtccgt tatcaacttg gccaacatga ggatcaccca tgtctgcagg
      241 gccaagtggc accgagtcgg tgcttttttt ggatcctagg tcttgaaagg agtgggaatt
      301 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
      361 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      421 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      481 atagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggcgtacggc
      541 caccatgaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga cgaaggacaa
      601 gaagtacagc atcgggctgg gcatcggcac caactctgtg ggctgggacg tgatcacgaa
      661 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      721 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      781 gctgaagaga accgccagaa gaagatacac cagactgtag gaaccggatc tgctatctgc
      841 aagagatctt cagccaacga gatggcccan gttggacgac agccttcttc cacagaactg
      901 gaagaagtcc tttcctggtt ctgagaagga ttagaaagcc tcgagcgggc accccaatct
      961 ttcggctacg ttcgtggtac gaaggtgtcc taacagcgag gagttacgcc agccatcctc
     1021 cancctggaa aaacaaagct ggggggaaaa gcacccgatc aaggccgaac ctggggtgtt
     1081 agaactaatc taggnccctg gcgc
//
