LOCUS       sequence-223303-        1396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs7903146 (TCF7L2 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            rs790314...
FEATURES             Location/Qualifiers
     source          1..1396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     18..37
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        133..151
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        203..221
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        308..519
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             536..583
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 attatngttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
       61 tggctttata tatcttgtgg aaaggacgaa acaccgacga gaaaccacag ttacagtttt
      121 agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt aaaataaggc
      181 tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc caagtggcac
      241 cgagtcggtg ctttttttgg atcctaggtc ttgaaaggag tgggaattgg ctccggtgcc
      301 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      361 aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      421 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      481 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg cgtacggcca ccatgaaaag
      541 gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aaggacaaga agtacagcat
      601 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      661 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      721 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
      781 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      841 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      901 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
      961 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
     1021 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
     1081 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
     1141 gctggtgcag acctacaacc ggctgttcga naaaacccca tcaacgccan cgggcgggga
     1201 acgccaaggg ccatcctggt cggccagaat gaagnagaac caaacggctg gaaaannctg
     1261 aatccccaac ttgccccggn agaaaaaaaa atgggcctgg ntccggccaa cctgaattgc
     1321 ctcggaaccc tgggcccgga ccccccaact tcaaaaaaac aatttccaac cgnggcccaa
     1381 aaangccaan atgggc
//
