LOCUS       sequence-223305-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            rs112576...
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        34..109
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggacgaaaca ccggtagaca cttgccccga gtcgttttag agctagaaat agcaagttaa
       61 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgaatt
      121 cgctagctag gtcttgaaag gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
      181 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
      241 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
      301 gccccccggc gacgcttcct gctccgcccc taantcggga aggttccttg cggttcgcgg
      361 cgtgccggac gtgacaaacg naanccncac gtctcactag taccctcgca nacggacagc
      421 gccnnngagc aatggnnncn cgccgaccgn gnngngctgn ggccaatanc ggctgctcan
      481 canggcgcgc cnagagcagc ggccgggaag gngcgnnnnn ngaggcngng tgtggnnnnn
      541 nnnngggtnn nnnagngnnn nnnnngnaga agnnnnnnan nnnggnngnn nngggnnngg
      601 gnngngaann nncnnnagnn nnnganangg nngnggnnng nnnnnnngnn ngnnagaaga
      661 nnnnnnanan nnnnngng
//
