LOCUS       sequence-223307-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            rs112576...
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        44..119
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             565..577
                     /estimated_length=13
     gap             579..593
                     /estimated_length=15
     gap             633..643
                     /estimated_length=11
     gap             665..674
                     /estimated_length=10
     gap             697..716
                     /estimated_length=20
ORIGIN
        1 cttgtggaaa ggacgaaaca ccggggcatg aaaccatctc cgtgttttag agctagaaat
       61 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
      121 tttttgaatt cgctagctag gtcttgaaag gggttggggt tgcgcctttt ccaaggcagc
      181 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
      241 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
      301 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
      361 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
      421 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
      481 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
      541 tgtggngcgn nnnnnngaan cnaannnnnn nnnnnnngnn nnnnnnnnnn nnnacnnnnn
      601 gnnngngnnn nnnantnnan nannannnnn nannnnnnnn nnnannnann nntnnnnnnn
      661 naaannnnnn nnnnanannn nngnnnnann nnntgtnnnn nnnnnnnnnn nnnnnnannn
      721 tnnngannnt nnnntnnnnn acnnt
//
