LOCUS       sequence-223309-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated repression of human islet enhancer containing 
            T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
            rs112576...
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        48..123
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gap             553..563
                     /estimated_length=11
     gap             565..581
                     /estimated_length=17
     gap             583..608
                     /estimated_length=26
     gap             610..626
                     /estimated_length=17
     gap             649..666
                     /estimated_length=18
     gap             739..752
                     /estimated_length=14
     gap             782..795
                     /estimated_length=14
     gap             873..883
                     /estimated_length=11
     gap             888..898
                     /estimated_length=11
ORIGIN
        1 tntnnntnng gnnnggacga aacaccggaa tatgacacaa cttccaggtt ttagagctag
       61 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      121 tgcttttttg aattcgctag ctaggtcttg aaaggggttg gggttgcgcc ttttccaagg
      181 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
      241 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
      301 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
      361 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
      421 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
      481 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
      541 ggggtgtgnn ncnnnnnnnn nnnannnnnn nnnnnnnnnn ngnnnnnnnn nnnnnnnnnn
      601 nnnnnnnngn nnnnnnnnnn nnnnnntcgn nnanananng aannannann nnnnnnnnnn
      661 nnnnnnantn ngcnananng nnnnnnnnna nnannnnnnn nnanannnaa nnngannnnn
      721 naannnggtg gnnnnnnann nnnnnnnnnn nnannnnnnn nnannnnnnn tnnnnnnnna
      781 tnnnnnnnnn nnnnngnann anannnnnnn nannggnnna nntnnnnnnn nnggnnaggn
      841 angntnnnnn gnngnnnang nnnnnannng nannnnnnnn nnngnaannn nnnnnnnnan
      901 nnnnntacnn agg
//
