LOCUS       sequence-223320-        3669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SC module. Thermostable beta-galactosidase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Geddes BA, Mendoza-Suarez MA, Poole PS
  TITLE     A Bacterial Expression Vector Archive (BEVA) for Flexible Modular 
            Assembly of Golden Gate-Compatible Vectors.
  JOURNAL   Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. 
            eCollection 2018.
  PUBMED    30692983
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2019-01-14"; volume: "9:3345. doi"; pages: " 
            10.3389/fmicb.2018.03345. eCollection 2018"
COMMENT     Sequence Label: pOGG050
FEATURES             Location/Qualifiers
     source          1..3669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1544..1561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1715..2303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1795..1814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2474..3265)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3487..3591)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(3659..3669,1..8)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cacgaggccc tttcgtccac tgaagagcca cttcgtggtc tcaaagccta ctttcttgta
       61 acaaatttga ggtctgcgag gtgagctaat tcttctggaa tttctttttg agtggctatt
      121 tctctgaata ccagggcgct tggccttaaa tatctcttct ttgtctcgaa atccacgtaa
      181 accaatccaa atctcatcct gaacccttgg gcccattcgt agttgtctgt tagtgaccag
      241 tggagatacc ctctaacatc agcaccttct ttcatagcat tgtaaactgc ctttagatgg
      301 cttacgagat agtgtggcct gtatctatct gctgcatcgg ccataccgtt ctctgtaatt
      361 atcattggta gctcgtaggc attgtttaaa tacttaagaa ggttctcaag gccctctggg
      421 tacatttccc atccaaagtc actagcaggt ctgcctgact ttgcaaatcc tcctctctca
      481 ctcataaatc catatccagg taaaggaact aggtgtccat ctttggctcc atataccagc
      541 ctggagtagt agtttacgcc gatccagtct agctttcctt ttgagtgtag aattgttaca
      601 aactcatagt ctttctttct gatttcctct acttcatcct tatactcctc cgctagagga
      661 tcgtgccaag caaaggcgta tatcactccc acggattttt ctgaatactc ttttatggca
      721 tcatatgctc cgatgtgagc ctgaattaag ttgaattttg ccttttctgc tgcttcaaag
      781 cttagatatc ctggtggaaa tcctgaacgt agattaatgt aaccttgatt gtagactacg
      841 tttggttcgt tcattgtgct ccacatgtca acgaggtcat caaggtgata agcaacgaag
      901 gcggcaaact tcacaaactc taccactgtt ttctcatcta accatcctgc aggagcccta
      961 tccgggccaa gtttccttac tgcaattggg tcatgaatcc ataatggaag aggccagtgg
     1021 tagaggttta atatgaaggt tttgcccctc tccttccagt ctgagtaaat cttgcgataa
     1081 tgttcaaggg cctccatgtt ggcaattttc tctagctctt ttattgtact ctctggaacg
     1141 tctacggaaa ttatgttgcc ttcttcatcc ttttccacat caactttaac gtcaaatgtt
     1201 ggctttggaa aaattcttgc ccactcaatg ccacctctaa tacaatccat tcctagcttt
     1261 tctgcaatgt catgatcttg cttatagagg tgccaatatg ctgggccgtt ctctggtaga
     1321 tctccactta ctagacctga tgctatgttc tccttgtcgt gaacccacac ccaccagtcg
     1381 ctttccactt cacttcctgg cagtcccatc tcaaactgga aaccagacca agaatatcca
     1441 aacatgaagt tttttgggaa cttcatttga gaccacagag tgattaatga atcggccaac
     1501 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2341 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2401 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2461 cttggtctga cagttatttg ccgactacct tggtgatctc gccttttacg taatggacaa
     2521 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
     2581 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
     2641 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
     2701 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
     2761 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
     2821 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
     2881 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
     2941 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
     3001 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
     3061 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
     3121 caatatcgct gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
     3181 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
     3241 cttcggcgat caccgcttcc ctcatgatgt ttaactttgt tttagggcga ctgccctgct
     3301 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
     3361 gcttggatgc ccgaggcata gactgtaccc caaaaaaaaa cagtcataac aagccatgaa
     3421 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     3481 gcgcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3541 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3601 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3661 taggcgtat
//
