LOCUS       sequence-223329-        7423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For broad gal4 inducible expression of Brainbow in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Pan YA, Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S,
            Ciruna B, Sanes JR, Lichtman JW, Schier AF
  TITLE     Zebrabow: multispectral cell labeling for cell tracing and lineage 
            analysis in zebrafish.
  JOURNAL   Development. 2013 Jul;140(13):2835-46. doi: 10.1242/dev.094631.
  PUBMED    23757414
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2013-07"; volume: "140"; issue: 
            "13"; pages: "2835-46"
COMMENT     Sequence Label: UAS:Zebrabow-B
FEATURES             Location/Qualifiers
     source          1..7423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(146..167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(517..528,529..691)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1124..1148
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1382..1406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1418..1451)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1460..1493)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1506..1515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1512..2213
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(1645..1663)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1913..1932
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2107..2130
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2343..2464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2547..2580)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      2594..2603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2600..2602,2603..2605,2606..3319)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    3442..3563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3570..3603)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3611..3620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3617..3619,3620..3622,3623..4336)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    4459..4580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(4953..5140,5141..5152)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5186..5202)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5186..5202)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5239..5259)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5239..5257)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5278..5294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5278..5294)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5291..5313)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5302..5318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5326..5332,5333..5350,5351..5356))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5371..5392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5509..5526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5680..6268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5760..5779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6439..7230,7231..7299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7062..7081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7300..7404)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
       61 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac gctggctaag
      241 aactcatcag cctccccggt ccatctaccc acgtaccaat gcaccaattg gccacaatga
      301 cggctactac atggtgccat tccttcctct ttataggaat ggagactacc tcctgtccaa
      361 caatgctctt ggatacgagt acgcctacct gttggaccca ggtcattgca caacaccaga
      421 aatgccctct gatctgcaaa agacgtgaat aatctgttca gacacccata tgcactctgt
      481 tccacacagg tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa
      541 attttactca agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag
      601 tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg
      661 agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt
      721 taatctcaaa aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt
      781 gtatttaatt atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt
      841 gtaaaacctc agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct
      901 agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga
      961 tgcgggaaga ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag
     1021 agactccctg gtgtctgaaa cacaggccag atgggccatt cgtacgctta atttcgtata
     1081 gggataacag ggtaatgata tcgatatcaa gggcccatct cgagcaagtt tgtacaaaaa
     1141 agcaggctcc gaattcctgc agcccggggg atcacgcggc catcaagctt aggcctccac
     1201 ggtggcttct aatccgtgag tcctagcggg tgacagccct ccgtcttcac aggcggagga
     1261 gagtcttccg tagggttcct cggagtactg tcctccgacg cgtgcaaggg tcgactctag
     1321 agggtatata atggatccca tcgcgtctca gcctcacttt gagctcctcc acacgaattc
     1381 gacccagctt tcttgtacaa agtgggctag cgagctcata acttcgtata ggatacttta
     1441 tacgaagtta taactcgaga taacttcgta tagcatacat tatacgaagt tataccggca
     1501 gatctgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg
     1561 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1621 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     1681 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     1741 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     1801 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     1861 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     1921 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     1981 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     2041 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     2101 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     2161 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagtaaagtg
     2221 tgcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2281 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2341 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2401 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2461 cttaaggcgt aaattgtaag cgttaatgct ccgagggccg ccaccacctg ttcctgtagg
     2521 aattggccgc gactctagga tctaggataa cttcgtatag gatactttat acgaagttat
     2581 tgatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2641 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2701 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2761 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg
     2821 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2881 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2941 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3001 cggcaacatc ctggggcaca agctggagta caacgccatc agcgacaacg tctatatcac
     3061 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     3121 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3181 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     3241 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3301 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
     3361 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3421 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3481 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3541 tccaaactca tcaatgtatc ttaatcgaga taacttcgta tagcatacat tatacgaagt
     3601 tataccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3661 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3721 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3781 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta
     3841 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3901 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3961 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4021 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4081 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4141 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4201 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     4261 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     4321 cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     4381 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     4441 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4501 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4561 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatccttagg gataacaggg
     4621 taatacgcgt gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg
     4681 gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt
     4741 gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg
     4801 acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata taatgtgaaa
     4861 acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag cgcaattcaa
     4921 ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt
     4981 tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta
     5041 agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat
     5101 aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc tggttcttga
     5161 ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc caccgcggtg
     5221 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     5281 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5341 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5401 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5461 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5521 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5581 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5881 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6121 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6301 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6361 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6421 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6481 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6541 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6601 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6661 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6721 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6781 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6841 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6901 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6961 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7021 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7081 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7141 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7201 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7261 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7321 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7381 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtg
//
