LOCUS       sequence-223331-       10689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For ubiquitous expression of Brainbow in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Pan YA, Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S,
            Ciruna B, Sanes JR, Lichtman JW, Schier AF
  TITLE     Zebrabow: multispectral cell labeling for cell tracing and lineage 
            analysis in zebrafish.
  JOURNAL   Development. 2013 Jul;140(13):2835-46. doi: 10.1242/dev.094631.
  PUBMED    23757414
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2013-07"; volume: "140"; issue: 
            "13"; pages: "2835-46"
COMMENT     Sequence Label: Ubi:Zebrabow
FEATURES             Location/Qualifiers
     source          1..10689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(146..167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(517..528,529..691)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1124..1148
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4647..4671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4684..4717)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(4726..4759)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4772..4781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4778..5479
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(4911..4929)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5179..5198
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5373..5396
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5609..5730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5813..5846)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      5860..5869
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5866..5868,5869..5871,5872..6585)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    6708..6829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6836..6869)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6877..6886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6883..6885,6886..6888,6889..7602)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    7725..7846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8219..8406,8407..8418)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8452..8468)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8452..8468)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8505..8525)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8505..8523)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8544..8560)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8544..8560)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8557..8579)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8568..8584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8592..8598,8599..8616,8617..8622))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8637..8658
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8775..8792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8946..9534)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9026..9045)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9705..10496,10497..10565))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10328..10347
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10566..10670)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
       61 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac gctggctaag
      241 aactcatcag cctccccggt ccatctaccc acgtaccaat gcaccaattg gccacaatga
      301 cggctactac atggtgccat tccttcctct ttataggaat ggagactacc tcctgtccaa
      361 caatgctctt ggatacgagt acgcctacct gttggaccca ggtcattgca caacaccaga
      421 aatgccctct gatctgcaaa agacgtgaat aatctgttca gacacccata tgcactctgt
      481 tccacacagg tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa
      541 attttactca agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag
      601 tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg
      661 agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt
      721 taatctcaaa aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt
      781 gtatttaatt atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt
      841 gtaaaacctc agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct
      901 agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga
      961 tgcgggaaga ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag
     1021 agactccctg gtgtctgaaa cacaggccag atgggccatt cgtacgctta atttcgtata
     1081 gggataacag ggtaatgata tcgatatcaa gggcccatct cgagcaagtt tgtacaaaaa
     1141 agcaggctac cagcaaagtt ctagaatttg tcgaaacatt tatgttatat atttcctgaa
     1201 aaaaattctg agtaagttct taagtgttat tgccagcaac ataaacaaca gacggcaaaa
     1261 tgaataaatg ataacaaagc agtaggctta aataaaccta atttttatag gctgttctct
     1321 acaaccctca aacagtgatt agttttgtac ttataaactt gccctttcat tcatatttca
     1381 agaaaattgg ttcagaagat ctggatattc tagcagttgt tcaagctcat ggagggatca
     1441 gtgacctgat tccacaatga ctaggcctaa tccagaaatt agatgactgt caacataaaa
     1501 aggcacagca ctcactagct gccctatata ttttattata ttttacatat attattttat
     1561 ttatttagct ctgagtgctg tactttctgg ttaaagaaaa ctgcttacaa cagctaacct
     1621 gtactacctc aggctcaggg aatttggaac aggtttgtct ggtttgtttc tttaaccatg
     1681 catgcttgtt ttcaactatg gcaacacagt cacatgggac attacagaaa tgatttgtcg
     1741 atgacatgcg acttttcttt aataaagcgc aaagatccca aaaagcaaac ttttaacaaa
     1801 aatcatataa ttatattttc aatccagctt tgtagcaact ttgtgctgct gttcactcag
     1861 caacagatag tcagtataag gtcagtgtgt ctcaaagcag tgccatctgt ttcacacatt
     1921 gcgttctata tataagtgtg ctggttgaca cgacactgta taaggcctag gctaaaacac
     1981 aaacaatgta gaatgacact gtgttttttt tgtaaacaaa tgttgttttt ggttaaacat
     2041 ctttgtgaaa acatcctcct gtcatgtatt tgctatattc aaatgttaaa cccgtgcaga
     2101 atagaacata tacaaaaaaa aacaacacaa cacattttta aacattatta aatatcaagt
     2161 attgctggca gttctgtttc tgttttacag taccctttgc cacagttctc cgcttttcct
     2221 ggtccagatt ccacaagtct gattcaccaa tagcaaagcg aataaacaac caaagcagcc
     2281 aatcactgct tgtagactgt cctgcgagac cggcccattc cagcacattc tggaaacttc
     2341 ctttatatga taattataaa tacatttaaa ttattgatac aaaacatgta attcctagaa
     2401 cataaccata gcaatcatta gttttcaggg taattatgta tttttaggat ttgactgcgg
     2461 aaagatctgg tcatgtgacg tctcatgaac gtcacggccc tgggtttcta taaatacagt
     2521 aggactctcg accatcggca gatttttcga agaagaagat cagtttcagg agccgtactg
     2581 ttccgtttca acgcaaatat taacggtaag agcgaatttc ctagtttgtt ttcatgccat
     2641 tctttaaaac catagcgtat tactttaatt atagtaaact ttcgctttct ttattacaag
     2701 agacgttttg tgttgattct ccgcggacat tttcggtcag acaatcagaa aatgaccgcg
     2761 gaggaccagt aacttgcatt acacgtaagt taaatcttcg tgtattaaaa tggttaggtt
     2821 gttaacgtca aataggttac cgtgtttgcg tgtgatcagg ttggttttgt tagatttttg
     2881 tcagtatttt taatttattt gttttagttt atttattttt tttgctgaat catagtttgt
     2941 gaacaaagaa cccggatgtt acatacagta cagccgccat gttacagaga gttataactt
     3001 aatcatttta aaaataattt tgccttactt ttagtttgtc atgttgagaa atgaggaaat
     3061 gttaaaatga ggaaatatcc aattaattta atatatcaaa ataatccatg attacaatgc
     3121 actgaactgg agaaaattaa gatgttttct agtgtcatga aacaaatgta agagatgtac
     3181 attgtagatg ttttatgtca agaattggct agttgatgca gcatactggc gatactcagt
     3241 tgtaataaca gtaacgttac atgttaatag actactgagt atgctgttct gtctatgtat
     3301 gctctgtaag ctacgagaag gactttttta aacagtaaag ggtgcaatat ttttacaaat
     3361 tgaattaaat aaaggctgtc tattaagtaa tatgcttgat atttttctta cttgatcgga
     3421 aataagaaaa aatataaacg ttgttgctct aaaaatccta gttcagttta gccaaccaca
     3481 aatacctttt tgttcctcca acagtttttt tttcttctct ataatatttg gcagtctata
     3541 gtactccaaa tgtttcccca cagtctaact aattggtaca gccaaaatca tgacacttat
     3601 tgcaataata attttggttc attggcattg ttgatagcct gtgccactaa tatggtcgat
     3661 tgatcatgct tcaggaagaa aactatattg tttgatgtaa gattattaaa tcttcacctg
     3721 cttccattac aaactattcc catcttattg aattctggta tgtcttaaag gattagttca
     3781 cttcccaaat caaaatttac ttagcctttt ttcatccatg atcccttttt ttcatcatta
     3841 atgaagaaat tgtttttgaa aaagtttcaa gatttttttc tctatattgt ggagcttgtt
     3901 agtttaaaat tccaaaatgc aatatgtggc ttcaaatggt tctaaatgat cccagtcaag
     3961 gaataacagt cttatctaat gaaaccatta gacctttaaa aataaaaaaa ataaaagtat
     4021 ttatttttaa atgactgagt gattaagttg aatttcagcg tttccttact gtgtagaagt
     4081 ccttccttac tggccccacc ctttggttct ctgccaatct gctacctaat gtaatgttgt
     4141 ggaacattat tattctttat ttcttaattt tttatttttt attttaaaac aatgtaaact
     4201 gcacagatgt gcagtttgtt taaaatggcc aatgctttgg aaatgcatga cataattaga
     4261 tttcatgatg cacaaagcca aatctcagag cttgtgcaaa atgagctatc atttcactag
     4321 gtaagaccct aaattttcat ataggatcat ttggacaatt ttgctgcagg taaaatgcat
     4381 tctatagtcc actgtcagcc attgttttgg atagtattta tttttctcta caagtatagt
     4441 caatagtttt ctattatttt aaaggtttgt aacatttaag ggtgaccaaa tgcaaagtaa
     4501 aatttcattt tcgggtgaac tatctcgttt aacatgggag aagtgcaaaa catacattat
     4561 tggctagaac attgtagtat tttttaaatg gaaatgtgtg attgctaatc ttactttgaa
     4621 tttgtttaca gggatccttc tcgagcaccc agctttcttg tacaaagtgg tgctagcgag
     4681 ctcataactt cgtataggat actttatacg aagttataac tcgagataac ttcgtatagc
     4741 atacattata cgaagttata ccggcagatc tgccaccatg gtgagcaagg gcgaggaggt
     4801 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     4861 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4921 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     4981 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     5041 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     5101 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     5161 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     5221 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     5281 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     5341 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     5401 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
     5461 catggacgag ctgtacaagt aaagtgtgcg gccgcgactc tagatcataa tcagccatac
     5521 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     5581 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     5641 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5701 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatgctccga
     5761 gggccgccac cacctgttcc tgtaggaatt ggccgcgact ctaggatcta ggataacttc
     5821 gtataggata ctttatacga agttattgat ccaccggtcg ccaccatggt gagcaagggc
     5881 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5941 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     6001 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     6061 acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     6121 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     6181 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     6241 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     6301 gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     6361 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     6421 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     6481 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     6541 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     6601 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     6661 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     6721 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6781 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa tcgagataac
     6841 ttcgtatagc atacattata cgaagttata ccggtcgcca ccatggtgag caagggcgag
     6901 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     6961 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     7021 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
     7081 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     7141 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     7201 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     7261 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     7321 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     7381 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     7441 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     7501 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     7561 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     7621 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     7681 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     7741 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7801 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta
     7861 agcgttaatc cttagggata acagggtaat acgcgtgatc tgcgaagata cggccacggg
     7921 tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat
     7981 acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg
     8041 tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg
     8101 tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa
     8161 ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa
     8221 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
     8281 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
     8341 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
     8401 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta
     8461 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     8521 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8581 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8641 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8701 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8761 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8821 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8881 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8941 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9001 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9061 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9121 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9181 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9241 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9301 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9361 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9421 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9481 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9541 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9601 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9661 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9721 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9781 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9841 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9901 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9961 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10021 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10081 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10141 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10201 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10261 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10321 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10381 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10441 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10501 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10561 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10621 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10681 cgaaaagtg
//
