LOCUS       sequence-223332-        8847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For cloning promoter into a Zebrabow vector with Gateway reaction. 
            Only compatible with single site Gateway. Not compatible with 
            Tol2-kit..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Pan YA, Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S,
            Ciruna B, Sanes JR, Lichtman JW, Schier AF
  TITLE     Zebrabow: multispectral cell labeling for cell tracing and lineage 
            analysis in zebrafish.
  JOURNAL   Development. 2013 Jul;140(13):2835-46. doi: 10.1242/dev.094631.
  PUBMED    23757414
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2013-07"; volume: "140"; issue: 
            "13"; pages: "2835-46"
COMMENT     Sequence Label: Zebrabow-GateDest
FEATURES             Location/Qualifiers
     source          1..8847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(146..167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(517..528,529..691)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1125..1249
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1274..1279,1280..1297,1298..1304)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1358..2017
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1424..1443)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2359..2664
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2543..2562
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2705..2829)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2842..2875)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2884..2917)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2930..2939
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2936..3637
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(3069..3087)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3337..3356
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3531..3554
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3767..3888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3971..4004)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      4018..4027
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4024..4026,4027..4029,4030..4743)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    4866..4987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4994..5027)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5035..5044
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5041..5043,5044..5046,5047..5760)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    5883..6004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6377..6564,6565..6576)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6610..6626)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6610..6626)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6663..6683)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6663..6681)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6702..6718)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6702..6718)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6715..6737)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6726..6742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6750..6756,6757..6774,6775..6780))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6795..6816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6933..6950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7104..7692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7184..7203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7863..8654,8655..8723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8486..8505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8724..8828)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
       61 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac gctggctaag
      241 aactcatcag cctccccggt ccatctaccc acgtaccaat gcaccaattg gccacaatga
      301 cggctactac atggtgccat tccttcctct ttataggaat ggagactacc tcctgtccaa
      361 caatgctctt ggatacgagt acgcctacct gttggaccca ggtcattgca caacaccaga
      421 aatgccctct gatctgcaaa agacgtgaat aatctgttca gacacccata tgcactctgt
      481 tccacacagg tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa
      541 attttactca agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag
      601 tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg
      661 agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt
      721 taatctcaaa aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt
      781 gtatttaatt atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt
      841 gtaaaacctc agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct
      901 agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga
      961 tgcgggaaga ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag
     1021 agactccctg gtgtctgaaa cacaggccag atgggccatt cgtacgctta atttcgtata
     1081 gggataacag ggtaatgata tcgatatcaa gggcccatct cgagacaagt ttgtacaaaa
     1141 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     1201 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
     1261 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     1321 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     1381 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     1441 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     1501 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     1561 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     1621 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     1681 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     1741 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     1801 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     1861 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     1921 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     1981 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa
     2041 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga
     2101 tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca
     2161 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg
     2221 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa
     2281 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg
     2341 agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat
     2401 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc
     2461 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     2521 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     2581 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     2641 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
     2701 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     2761 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
     2821 caaagtggtg ctagcgagct cataacttcg tataggatac tttatacgaa gttataactc
     2881 gagataactt cgtatagcat acattatacg aagttatacc ggcagatctg ccaccatggt
     2941 gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc
     3001 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3061 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     3121 gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga
     3181 ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3241 cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa
     3301 ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
     3361 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     3421 ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta
     3481 catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat
     3541 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca
     3601 ccacctgttc ctgtacggca tggacgagct gtacaagtaa agtgtgcggc cgcgactcta
     3661 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3721 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3781 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     3901 taagcgttaa tgctccgagg gccgccacca cctgttcctg taggaattgg ccgcgactct
     3961 aggatctagg ataacttcgt ataggatact ttatacgaag ttattgatcc accggtcgcc
     4021 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     4081 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     4141 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     4201 accctcgtga ccaccctgac ctggggcgtg cagtgcttcg cccgctaccc cgaccacatg
     4261 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     4321 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     4381 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     4441 cacaagctgg agtacaacgc catcagcgac aacgtctata tcaccgccga caagcagaag
     4501 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     4561 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4621 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     4681 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4741 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     4801 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     4861 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4921 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4981 tatcttaatc gagataactt cgtatagcat acattatacg aagttatacc ggtcgccacc
     5041 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5101 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5161 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5221 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     5281 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5341 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5401 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5461 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     5521 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     5581 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5641 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     5701 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     5761 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     5821 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     5881 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5941 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6001 cttaaggcgt aaattgtaag cgttaatcct tagggataac agggtaatac gcgtgatctg
     6061 cgaagatacg gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca
     6121 gctgctgatg aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc
     6181 ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc
     6241 aaaggaccaa tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca
     6301 gaataaataa catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc
     6361 aagggaaaat agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat
     6421 tgtaaggagt aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta
     6481 ttgattttta attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt
     6541 aatcaagtaa aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca
     6601 agcccagcag atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     6661 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     6721 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6781 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6841 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6901 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7501 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7861 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7981 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8821 gttccgcgca catttccccg aaaagtg
//
